Mutant aprE promoter

Details Mutant aprE promoter Mutant aprE promoter

2005-06-28

2005-06-28

Leffers, Gerry (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S252300, C435S471000, C435S320100, C536S023100, C536S024100, C530S350000

Reexamination Certificate

active

06911322

ABSTRACT:
The present invention provides a mutant aprE promoter and methods for the production of a desired protein in aBacillushost cell, which comprises the mutant aprE promoter. The present invention provides the sequence of a preferred aprE mutant promoter, which provided for a 100-fold increase in the production of a protein fromBacillus subtilis.

REFERENCES:
patent: 4828994 (1989-05-01), Fahnestock et al.
patent: 5922586 (1999-07-01), Outtrup et al.
patent: 6509185 (2003-01-01), Valle et al.
Park, et al. Journal of Bacteriology, May 1989, vol. 171, No. 5, pp. 2657-2665.
Jan, et al. FEMS Microbiology Letters, Jan. 2000, vol. 183, pp. 9-14.
Ferrari, et al. Journal of Bacteriology, Jan. 1988, vol. 170, No. 1, pp. 289-295.
*Bakhiet, Nouna and Donald P. Stahly, “Studies on Transfection and Transformation of Protoplasts ofBacillus larvae, Bacillus subtilis, andBacillus popilliae,” Applied and Environmental Microbiology, pp. 577-581, Mar. 1985.
*Bolanos, Victor, “Sobreproduccion de la Enzima Beta-Galactosidasa deEscherichia colienBacillus subtilis,” Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, 1994.
*Bron, Sierd,Molecular Biological Methods for Bacillus, ed. Harwood and Cutting, John Wiley & Sons, pp. 75-174, 1990.
*Chang, Shing et al., “High Frequency Transformation ofBacillus subtilisProtoplasts,” Molec. Gen. Genet., vol. 168, pp. 111-115, 1979.
*Contente, Sara et al., “Marker Rescue Transformation by Linear Plasmid DNA inBacillus subtilis,” Plasmid, vol. 2, pp. 555-571, 1979.
*Debabov, V.G., “The Industrial Use of Bacilli,”The Molecular Biology of the Bacilli, Dubnau, D.A., ed., Academic Press, vol. 1, pp. 331-370, 1982.
*Fischer, Hans-Martin, et al., “Introduction of plasmid pC194 intoBacillus thuringiensisby protoplast transformation and plasmid transfer,” Arch. Microbiol., vol. 139, pp. 213-217, 1984.
*Genex Corp., Sequence Accession No. N60475, Database EMBL Online!, “Sequence of the apr 'Bsu! gene encoding subtilisin” XP-002166627, Aug. 24, 1991.
*Haima, Peter, et al., “Novel plasmid marker rescue transformation system for molecular cloning inBacillus subtilisenabling direct selection of recombinants,” Mol. Gen. Genet, vol. 223, pp. 185-191, 1990.
*Henner, Dennis et al., “Localization ofBacillus subtilissacU(Hy) Mutations to Two Linked Genes with Similarities to the Conserved Procaryotic Family of Two-Component Signalling Systems,” Journal of Bacteriology, vol. 170, No. 11, pp. 5102-5109, Nov., 1988.
*Henner, Dennis, et al., “Location of the Targets of the hpr-97, sacU32(Hy), and sacQ36(Hy) Mutations in Upstream Regions of the Subtilisin Promoter,” Journal of Bacteriology, vol. 170, No. 1, pp. 296-300, Jan, 1988.
*Holubova, I., et al., “Transfer of Liposome-Encapsulated Plasmid DNA toBacillus subtilisProtoplasts and Calcium-TreatedEscherichia coliCells,” Folia Microbiol. vol. 30, pp. 97-100, 1985.
*Kunst, F., et al., “The complete genome sequence of the Gram-positive bacteriumBacillus subtilis,” Nature, vol. 390, Nov. 20, 1997.
*Mann, Stephen P., et al., “Transformation of Bacillus spp.: an Examination of the Transformation of Bacillus Protoplasts by Plasmids pUB110 and pHV33,” Current Microbiology, vol. 13, pp. 191-195, 1986.
*McCready, Paula M., et al., “Bacillus subtilisSenS exerts its activity through a site in the 5′ flanking region of the aprE promoter,” Journal of General Microbiology, vol. 138, pp. 2069-2074, 1992.
*McDonald, Karen Orzech, et al., “Plasmid Transformation ofBacillus sphaericus1593,” Journal of General Microbiology, vol. 130, pp. 203-208, 1984.
*Merino, Enrique, et al., “BioFeedback, A General, PCR-Based Method for Single or Combinatorial Oligonucleotide-Directed Mutagenesis on pUC/M13 Vectors,” BioTechniques, vol. 12, No. 4, pp. 508-510, 1992.
*Olmos, J., et al., “A functional SpoOA is required for maximal aprE expression inBacillus subtilis, FEBS Letters,” vol. 381, pp. 29-31, 1996.
*Oyama, Hiroshi, et al., “Secretion ofEscherichia coliAminopeptidase P inBacillus subtilisUsing the Prepro-Structure Coding Region of Subtilisin Amylosacchariticus, Journal of Fermentation and Bioengineering,” vol. 68, No. 4., pp. 289-292, 1989.
*Papas, Takis, S., et al., ed.Gene Amplification and Analysis, Elsevier, vol. 3, pp. 64-87, 1983.
*Perego, Marta, et al., “Sequence Analysis and Regulation of the hpr Locus, a Regulatory Gene for Protease Production and Sporulation inBacillus subtilis,” Journal of Bacteriology, vol. 170, No. 6, pp. 2560-2567, Jun., 1988.
*Ross, Wilma, et al., “A Third Recognition Element in Bacterial Promoters: DNA Binding by the α Subunit of RNA Polymerase,” Science, vol. 262, pp. 1407-1413, Nov. 26, 1993.
*Sanger, F., et al., “DNA sequencing with chain-terminating inhibitors,” Proc. Natl. Acad. Sci. USA, vol. 74, No. 12, pp. 5463-5467, Dec., 1977.
*Smith, Michael D., et al., “Protoplast Transformation in Coryneform Bacteria and Introduction of an α-Amylase Gene fromBacillus amyloliquefaciensinto Brevibacterium lactofermentum,” Applied and Environmental Microbiology, vol. 51, No. 3, pp. 634-639, Mar., 1986.
*Sonenshein, Abraham L., “Metabolic Regulation of Sporulation and Other Stationary-Phase Phenomena,”Regulation of Procaryotic Development, Smith, I. et al, ed., American Society for Microbiology, Washington, D.C., pp. 109-130, 1989.
*Stahl, Mark L., et al., “Replacement of theBacillus subtilisSubtilisin Structural Gene with an In Vitro-Derived Deletion Mutation,” Journal of Bacteriology, vol. 158, No. 2, pp. 411-418, May, 1984.
*Valle, Fernando, et al., “Subtilisin: a Redundantly Temporally Regulated Gene?” Regulation of Procaryotic Development, Smith, I. et al., ed., American Society for Microbiology, Washington, D.C. pp. 131-146, 1989.
*Vorobjeva, I.P., et al., “Transformation of Bacillus Megaterium Protoplasts by Plasmid DNA,” FEMS Microbiology Letters, vol. 7, pp. 261-263, 1980.
*Walsh, Kenneth, et al., “Characterization of rate-controlling steps in vivo by use of an adjustable expression vector,” Proc. Natl. Acad. Sci. US.A., vol. 82, pp. 3577-3581, Jun., 1985.
*Weinrauch, Y., et al., “Plasmid Marker Rescue Transformation inBacillus subtilis,” Journal of Bacteriology, vol. 154, No. 3, pp. 1077-1087, Jun., 1983.
*Weinrauch, Y., et al., “Plasmid Marker Rescue Transformation Proceeds by Breakage-Reunion inBacillus subtilis,” Journal of Bacteriology, vol. 169, No. 3, pp. 1205-1211, 1987.
*Wong, Sui-Lam, et al., “The subtilisin E gene ofBacillus subtilisin transcribed from a σ37promoter in vivo,” Proc. Natl. Acad. Sci. USA, vol. 81, pp. 1184-1188, Feb., 1984.
*Yang, Maria Y., et al., “Cloning of the Neutral Protease Gene ofBacillus subtilisand the Use of the Cloned Gene to Create an In Vitro-Derived Deletion Mutation,” Journal of Bacteriology, vol. 160, No. 1, pp. 15-21, Oct., 1984.
*Zukowski, Mark M., “Production of Commercially Valuable Products,”Biology of bacilli: applications to industry, Doi, R.H., et al., ed., Butterworth-Heinemann, Stoneham, Massachusetts, pp. 311-337, 1992.

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