Mutant .alpha.-amylase having introduced therein a disulfide bon

Details Mutant .alpha.-amylase having introduced therein a disulfide bon Mutant .alpha.-amylase having introduced therein a disulfide bon

1997-07-11

1999-12-28

Sisson, Bradley L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435202, 435203, 435204, 435205, 435262, 435267, 435274, 435275, 4353201, 536 232, C12P 1920, C12N 928, C07G 1700, C08B 100

Patent

active

060080264

ABSTRACT:
Novel .alpha.-amylase enzymes are disclosed in which one or more disulfide bonds are introduced into the enzyme via addition or substitution of a residue with a cysteine. The disclosed .alpha.-amylase enzymes show altered or improved stability and/or activity profiles.

REFERENCES:
Declerck et al. (Jan. 1995) Hyperthermostable mutants of Bacillus licheniformis a-amylases: multiple amino acid replacements and molecular modelling. Protein Engineering 8 (10): 1029-1037.
Schwermann et al. (Dec. 1994) Purification, properties and structural aspects of thermoacidophilic a-amylase from Alicyclobacillus acidocaldarius atcc 27009: Insight into acidostability of proteins. Eur. J. Biochem. 226 (3): 981-991.
Joyet et al. (Dec. 1992) Hyperthermostable variants of a highly thermostable alpha-amylase. Biotechnology 10: 1579-1583.

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