Mutant 2.5-diketo-L-gluconic acid reductases

Details Mutant 2.5-diketo-L-gluconic acid reductases Mutant 2.5-diketo-L-gluconic acid reductases

2008-05-20

2008-05-20

Pak, Yong D. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S440000, C435S069100, C435S071100, C435S252300, C435S320100, C530S350000, C536S023200

Reexamination Certificate

active

07374917

ABSTRACT:
Described herein are novel nucleic acids, proteins and methods that can be used to provide new catalysts with desirable traits for industrial processes. In particular, novel reductases isolated from the environment using PCR methods are described.

REFERENCES:
patent: 3922194 (1975-11-01), Sonoyama et al.
patent: 4179337 (1979-12-01), Davis et al.
patent: 4301144 (1981-11-01), Iwashita et al.
patent: 4496689 (1985-01-01), Mitra
patent: 4640835 (1987-02-01), Shimizu et al.
patent: 4670417 (1987-06-01), Iwasaki et al.
patent: 4791192 (1988-12-01), Nakagawa et al.
patent: 5795761 (1998-08-01), Powers et al.
patent: 5912161 (1999-06-01), Hurle et al.
patent: 6576452 (2003-06-01), Donnelly et al.
patent: 6864075 (2005-03-01), Donnelly et al.
patent: 87/00863 (1987-02-01), None
patent: 87/05330 (1987-09-01), None
Branden et al. (introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
Guo et al. Proc Natl Acad Sci USA. Jun. 22, 2004;101(25):9205-10.
Altschul et al., “Basic Local Alignment Search Tool,” J. Mol. Biol., V. 215, 1990, pp. 403-410.
Altschul et et al., “Gapped BLAST and PSI-BLAST: a new generation of protein database programs,” Nucl. Acids Res., vol. 25, pp. 3389-3402, 1997.
Altschul et al., “Basic Local Alignment Statistics, Methods in Enzymology”, V. 266, pp. 460-480 (1993).
Anderson et al., “Production of 2-Keto-L-Gulonate, an Intermediate in L-Ascorbate Synthesis, by a Genetically ModifiedErwinia herbicola,” Science, vol. 230, Oct. 11, 1985, pp. 144-149.
Aplin et al., “Preparation, Properties, and Applications of Carbohydrate Conjugates of Proteins and Lipids,”Crit. Rev. in Biochem.1981, pp. 259-306.
Ausubel, Frederick et al., “Short Protocols in Molecular Biology,”Current Protocols in Molecular Biology, 2nded., Greene Publishing Associates & John Wiley & Sons. New York, N.Y. 1992.
Ausubel et al., Ed.Current Protocols in Molecular Biology, John Wiley & Sons, Inc. Ch. 9, 1987.
Batzer et al., “Enhanced evolutionary PCR using oligonucleotides with inosine at the 3′-terminus”, Nucl. Acids. Res., vol. 19, p. 5081, 1991.
Blattner et al., “The Complete Genome Sequence ofEscherichia coliK-12,”Science, vol. 277, pp. 1453-1462, 1997.
Davey et al., “Ascorbate Biosynthesis in Arabidopsis Cell Suspension Culture,”Plant Physiol. vol. 121, pp. 535-543, 1999.
Devereux et al., “A Comprehensive set of sequence analysis programs for the VAX,”Nucl. Acids Res., vol. 12, p. 387-395, 1984.
Echenfeldt et al., “DNA from Uncultured Organisms as a Source of 2,5-Kiketo-D-Gluconic Acid Reductases,”Applied and Environmental Microbiology, V. 67 (9), Sep. 2001, p. 4206-4214.
Edge et al., “Deglycosylation of Glycoproteins by Trifluoromethanesulfonic Acid,”J. Med. Chem., vol. 30, pp. 1229-1239, 1987.
Evan et al., “Isolation of Monoclonal Antibodies Specific for Human c-myc Proto-Oncogene Product,”Mol. and Cell. Biology, vol. 5, pp. 3610-3616, 1985.
Field et al., “Purification of a RAS-Responsive Adenylyl Cyclase Complex fromSaccharomyces cerevisiaeby Use of an Epitope Addition Method,”Mol. and Cell. Biology, vol. 8, pp. 21592165, 1988.
Feng et al., “Progressive Sequence Alignment as a Prerequisite to Correct Phylogenetic Tress”J. Mol Evol.vol. 25, pp. 351-360, 1987.
Furste et al., “Molecular cloning of the plasmid PR4 primase region in a multi-host-range tacP expression vector,”Gene, vol. 48, pp. 119-131, 1986.
Goding, J., “Production of Monoclonal Antibodies,” Monoclonal Antibodies: Principle and Practice, 1986, pp. 59-103.
Grindley, J. F, “Conversion of Glucose to 2-Keto-L-Gulonate, an Intermediate in L-Ascorbate Synthesis, by a Recombinant Strain ofErwinia citreus,” Applied and Environmental Microbiology, vol. 54, No. 7, Jul. 1988, p. 1770-1775.
Hakimuddin et al., “A Chemical Method for the Deglycosylation of Proteins,”Archives of Biochem. And Biophysics, vol. 159, 1987, p. 52-57.
Higgins et al., “Fast and sensitive multiple sequence alignments on microcomputer,”CABIOS, vol. 5, 1989, p. 151-153.
Hopp et al., “A short polypeptide marker sequence useful for recombinant protein identification and purification,”Bio/Tech., vol. 6, 1988, p. 1204-1210.
Jez et al., “Comparative anatomy of the aldo-keto reductase superfamily,”Bioch. J., vol. 326, 1997, pp. 625-636.
Khurana et al., “Crystal structure of 2,5-diketo-D-gluconic acid reductase A complexed with NADPH at 2.1-A resolution,”Proc. Natl. Acad. Sci. USA, vol. 95, pp. 6768-6773, Jun. 1998 Biophysics.
Karlin et al., “Applications and statistics for multiple high-scoring segments in molecular sequences,” Proc. Natl. Acad. Sci. USA, vol. 90, pp. 5873-5877, Jun. 1993.
Kohler et al., “Continuous cultures of fused cells secreting antibody of predefined specificity,” Nature, vol. 256, pp. 495-497, 1975.
Laemmli U.K. “Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4,” Nature, vol. 227, pp. 680-685, 1970.
Lutz-Freyermuth, “Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA,” Proc. Natl. Acad. Sci. USA, vol. 87, pp. 6393-6397, 1990.
Maniatis et al., Molecular Cloning: A Laboratory Manual, 2d Edition, 1989.
Martin et al., “GAP Domains Responsible for Ras p21-Dependent Inhibition of Muscarinic Atrial K+ Channel Currents,”Science, vol. 255, (1992), pp. 192-194.
Miller, J. H., “Purification and Characterization of 2,5-Diketo-D-gluconate Reductase fromCorynebacteriumSp,”J. Biol. Chem., vol. 262, (1987), pp. 9016-9020.
Needleman et al., “A General Method Applicable to the Search for Similarities in the Amino Acid Sequence of Two Proteins,”J. Mol. Biol., vol. 48, pp. 443-453, 1970.
Nishikimi et al., “Biochemistry and Molecular Biology of Ascorbic Acid Biosynthesis,”Subcell. Biochemistry, pp. 17-39, 1996.
Ohtsuka et al., “An Alternative Approach to Deoxyoligonucleotides as Hybridization Probes by Insertion of Deoxyinosine at Ambiguous Codon Positions” (1985)J. Biol. Chem.vol. 260, No. 5, pp. 2605-2608.
Paborsky et al., “Mammalian cell transient expression of tissue factor for the production of antigen,” Protein. Engin. vol. 3, pp. 547-553, 1990.
Pearson et al., “Improved tools for biological sequence comparison,” Proc. Natl. Acad. Sci. USA, vol. 85, pp. 2444-2448, Apr. 1988.
Ratnam et al., “The Argine 276 Anchor for NADP(H) Dictates Fluorescence Kinetic Transients in 3á -Hydroxysteroid Dehydrogenase, a Representative Aldo-Keto Reductase,” Biochemistry, vol. 38, pp. 7856-7864 (1999).
Redenbach et al., “A set of ordered cosmids and a detailed genetic and physical map for the 8 MbStreptomyces coelicolorA3(2) chromosome,” Mol. Microb., vol. 21, pp. 77-96 (1996).
Rossolini et al., “Use of deoxyinosine-containing primers vs degenerate primers for polymerase chain reaction based on ambiguous sequence information” (1994)Mol Cell. Probes8, 91-98.
Sarkar et al., “Restriction-site PCR: A Direct Method of Unknown Sequence Retrieval Adjacent to a Known Locus by Using Universal Primers,”PCR Methods and Applic.vol. 2, 318-322 (1993).
Sambrook et al., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Press, 1989.
Seery et al., “Molecular Evolution of the Aldo-keto Reductase Gene Superfamily,”J. Mol. Evol., vol. 46, pp. 139-146 (1998).
Selenska et al., “DNA Recovery and Direct detection of Tn5 sequences from soil,”Letters in Applied Microbiol., vol. 13, pp. 21-24, (1991).
Skinner et al., “Use of the Glu-Glu-Phe C-terminal Epitope for Rapid Purification of the Catalytic Domain of

Affiliated with

Also associated with

Rating

Mutant 2.5-diketo-L-gluconic acid reductases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Mutant 2.5-diketo-L-gluconic acid reductases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mutant 2.5-diketo-L-gluconic acid reductases will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2794401