Mutan endonuclease with substrate-specific cleavage activity

Details Mutan endonuclease with substrate-specific cleavage activity Mutan endonuclease with substrate-specific cleavage activity

2006-10-02

2009-12-15

Ramirez, Delia M (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S019000, C435S069100, C435S320100, C435S325000, C435S252300, C530S350000, C536S023200

Reexamination Certificate

active

07632667

ABSTRACT:
A novel mutant endonuclease V not exhibiting a nonspecific nucleic acid cleavage activity but showing a specific activity. The present invention relates to a mutant endonuclease V not exhibiting a nonspecific nucleic acid cleavage activity but having a specific activity, to a gene coding for the endonuclease V, to a recombinant DNA containing the gene, to a transformant or transductant containing the recombinant DNA, and to a method for producing the endonuclease V. The present invention also relates to a method of using the endonuclease V for nucleic acid cleavage, and to a reagent kit for detection or modification of a nucleic acid or gene that contains the endonuclease V.

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