Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2006-05-02
2006-05-02
Nashed, Nashaat T. (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S155000, C435S232000
Reexamination Certificate
active
07037693
ABSTRACT:
The present invention provides a fatty acid lyase, wherein the activity of the lyase for 9-hydroperoxide substrates is greater than the activity for 13-hydroperoxide substrates and wherein Kmand Vmaxof the lyase for 9-hydroperoxylinolenic acid are greater than Kmand Vmaxof the lyase for 9-hydroperoxylinoleic acid. More particularly, the invention provides a lyase present in melon (Cucumis melo). The invention also provides a nucleic acid encoding the lyase, vectors, and expression systems with which the recombinant lyase can be obtained. The invention also provides methods of using the lyase of the invention, including methods of cleaving 9-hydroperoxylinoleic acid, 9-hydroperoxylinolenic acid, 13-hydroperoxylinoleic acid, and 13-hydroperoxylinolenic acid. Also, the invention provides a method of preparing 3-(Z)-nonenal, (3Z,6Z)-nonadienal, 2-(E)-nonenal, (2E,6Z)-nonadienal, or their corresponding alcohols and a method of preparing n-hexanal, 3-(Z)-hexen-1-al, 2-(E)-hexen-1-al, or their corresponding alcohols using the lyase of the present invention.
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Brash Alan
Tijet Nathalie
Whitehead Ian M.
Firmenich SA
Nashed Nashaat T.
Needle & ROsenberg, PC
Vanderbilt University
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