Muscle sample prepared for prion assay

Details Muscle sample prepared for prion assay Muscle sample prepared for prion assay

2007-01-23

2007-01-23

Le, Long V. (Department: 1641)

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

C435S001100, C435S007100, C435S007210, C435S023000, C435S287200, C435S380000, C435S962000, C530S412000, C530S419000, C436S517000, C436S524000, C436S528000, C436S534000, C436S536000, C436S538000, C436S017000, C436S166000, C436S174000, C436S175000, C436S177000

Reexamination Certificate

active

10211942

ABSTRACT:
A method of preparing a sample of muscle tissue and of assaying the prepared sample to determine the presence of prions in the sample is disclosed. The muscle tissue is homogenized and mixed with a complexing agent which forms a complex with a higher specific gravity than PrPSc, the complexing agent or other components of the homogenate. Gravity is then used (e.g. ultra centrifugation) to concentrate the complex and the concentrate is assayed to detect prions. The muscle tissue is preferably extracted from a muscle or group of muscles such as hind limb muscle which have a higher or more preferably the highest concentration of prions as compared to other muscle in the mammal.

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