Murine autonomous replication sequences and vectors containing

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4353201, 4351723, 4352402, 536 27, 935 42, 935 70, 935 71, 935 34, 935 82, C12P 2100, C07H 1512, A01K 6702, C12N 1585

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050810189

ABSTRACT:
The present invention provides a vector for the expression of heterologous proteins in mammalian cells, wherein it contains at least one first consensus sequence is homologous to the ##STR1## in at least 10 nucleotides in total and including the first three thereof and at least one second sequence which in one of the 6th, 8th, 9th and 10th nucleotide, as well as in at least 9 nucleotides, is homologous to the sequence ##STR2## or in at least 10 nucleotides is homologous to the sequence

REFERENCES:
Kaufman et al., Mol. Cell Biol., vol. 5, pp. 1750-1754, 1980, "Coamplification and Coexpression of Human Tissue-Type Plasminogen Activator and Murine Dihydrofolate Reductase Sequences, Chinese Hamster Ovary Cells".
Ariga et al., Mol Cell. Biol. vol. 7, No. 1, pp. 1-6, Jan. 1987, "Autonomous Replicating Sequences from Mouse Cells, which can Replicate in Mouse Cells in Vivo and in Vitro".
Ariga et al., Mol Cell. Biol., vol. 5, No. 3, Mar. 1985, pp. 563-568, "Cloned Mouse DNA Fragments can Replicate in a Simian Virus 40 T Antigen-Dependent System In Vivo and In Vitro".
Pulm et al., Mol. Cell. Biol. vol. 5, No. 2, pp. 295-304, Feb. 1985, "Transfection of Mouse Fibnblast Cells within Promoterless Herpes Simplex Virus Thymidine Kinase . . . ".
Sanae et al., EMBO J., vol. 6, No. 8, pp. 2365-2371, Aug. 1987, "Possible Function of the c-myc Product: Promotion of Cellular DNA Replication".
Holst et al., Cell, vol. 52, pp. 355-365, 1988, "Murine Genomic DNA Sequences Replicating Autonomously in Mouse L Cells".

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