Murd protein method and kit for identification of inhibitors

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435183, 435184, C12N 900, C12N 999

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active

056816944

ABSTRACT:
The invention provides isolated nucleic acid compounds encoding the murD stem peptide biosynthetic gene of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the MurD enzyme product and a method for identifying compounds that inhibit stem peptide biosynthesis.

REFERENCES:
Mengin-Lecreulx, et al., "Organization Of The murE-murG Region Of E. Coli", J. Bacteriol, 171:11, pp. 6126-6134 (1989).
M. Ikeda, et al., "Homology Among murC, murD, murE And murF Proteins In E. Coli And That Between E. Coli murG And A Possible murG Protein In B. Subtilis", J. Gen. Appl. Microbiol., 36, pp. 179-187 (1990).
Daniel et al. (1993) DNA sequence of the murE-murD region of Bacillus subtillis 168, J. Gen. Microbiol. 139 (2): 361-370 Feb. 1993.
Leach et al. (1994) Rhizobium meliloti homologs of Escherichia coli mur genes, Gene 148: 87-90 Oct. 11, 1994.
Fleischmann et al. (1995) Whole-genome random sequencing and assembly of Haemophilus influenzae Rd, Science 269: 496-498 and 507-512 Jul. 28, 1995.

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