Multizone analytical element having labeled reagent concentratio

Details Multizone analytical element having labeled reagent concentratio Multizone analytical element having labeled reagent concentratio

1985-08-28

1989-02-21

Castel, Benoit

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Calorimeter

422 57, 422 58, 435 7, 435805, 436807, 436810, 436815, G01N 3122, G01N 3353, G01N 33563

Patent

active

048063118

ABSTRACT:
A multizone test device for the determination of analyte from a liquid test medium upon contact with the liquid test medium and a labeled reagent comprising a chemical group having a detectable physical property. The test device preferably comprises multilayers including a reagent layer incorporated with an immobilized reagent and a detection layer incorporated with an immobilized form of a binding substance for the labeled reagent. The immobilized reagent and the labeled reagent comprise specific binding partners which will bind to each other dependent upon the amount of analyte present. Labeled reagent which does not become bound to the immobilized reagent in the reagent layer migrates into the detection layer and becomes bound to and immobilized by the immobilized binding substance therein. As a result, reverse migration of the labeled reagent into the reagent layer is prevented and the detectable physical property provided by the label of the labeled reagent is localized in the detection layer for the precise measurement thereof and correlation to the amount of analyte in the test medium.

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