Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2007-12-11
2007-12-11
Nashed, Nahsaat T. (Department: 1656)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S220000, C435S221000, C435S252300, C435S320100, C510S300000
Reexamination Certificate
active
10500943
ABSTRACT:
Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or re-combinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 ofBacillus amyloliquefacienssubtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 ofBacillus amyloliquefacienssubtilisin are also described.
REFERENCES:
patent: 5336611 (1994-08-01), van Eekelen et al.
patent: 5352603 (1994-10-01), Vetter et al.
patent: 5453372 (1995-09-01), Vetter et al.
patent: 5665587 (1997-09-01), Aaslyng et al.
patent: 5972682 (1999-10-01), Bott et al.
patent: 6287841 (2001-09-01), Mulleners et al.
patent: 6300116 (2001-10-01), von der Osten et al.
patent: 6312936 (2001-11-01), Poulose et al.
patent: 6376450 (2002-04-01), Ghosh et al.
patent: 6482628 (2002-11-01), Poulose et al.
patent: 6599730 (2003-07-01), Brode et al.
patent: 6605458 (2003-08-01), Hansen et al.
patent: 6610642 (2003-08-01), Ghosh et al.
patent: 6815193 (2004-11-01), Poulose et al.
patent: 6831053 (2004-12-01), Ghosh et al.
patent: 6838425 (2005-01-01), Ghosh et al.
patent: 6927055 (2005-08-01), Poulose et al.
patent: WO 99/20723 (1999-04-01), None
Bott Richard R.
Estell David A
Kellis, Jr. James T
Poulose Ayrookaran J.
Genencor International Inc.
MacKnight Kamrin T.
Moore William W.
Nashed Nahsaat T.
LandOfFree
Multiply-substituted protease variants does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Multiply-substituted protease variants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multiply-substituted protease variants will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3867332