Multiplexed fluorescent detection in microfluidic devices

Radiant energy – Luminophor irradiation

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C250S459100

Reexamination Certificate

active

06838680

ABSTRACT:
An optical detection and orientation device is provided comprising a housing having an excitation light source, an optical element for reflecting the excitation light to an aspherical lens and transmitting light emitted by a fluorophore excited by said excitation light, a focusing lens for focusing the emitted light onto the entry of an optical fiber, which serves as a confocal aperture, and a moveable carrier for accurately moving said housing over a small area in relation to a channel in a microfluidic device. The optical detection and orientation device finds use in detecting fluorophores in the channel during operations involving fluorescent signals.

REFERENCES:
patent: 5296703 (1994-03-01), Tsien
patent: 5324635 (1994-06-01), Kawase et al.
patent: 5459325 (1995-10-01), Hueton et al.
patent: 5571410 (1996-11-01), Swedberg et al.
patent: 5587128 (1996-12-01), Wilding et al.
patent: 5614726 (1997-03-01), Kaye et al.
patent: 5630924 (1997-05-01), Fuchs et al.
patent: 5730850 (1998-03-01), Kambara et al.
patent: 5741412 (1998-04-01), Dovichi et al.
patent: 5750015 (1998-05-01), Soane et al.
patent: 5833826 (1998-11-01), Nordman
patent: 5847400 (1998-12-01), Kain et al.
patent: 6614030 (2003-09-01), Maher et al.
patent: 20020015147 (2002-02-01), Maher et al.
patent: WO 9849543 (1998-11-01), None
patent: WO 0120309 (2001-03-01), None
Huang, C. et al. (1992). “DNA Sequencing Using Capillary Array Electrophoresis,”Analytical Chemistry64:2149-2154.
Khandurina, J. et al. (1999). “Microfabricated Poruous Membrane Structure for Sample Concentration and Electrophoretic Analysis,”Analytical Chemistry71(9):1815-1819.
Kheterpal, I. et al. (1996). “DNA Sequencing Using a Four-Color Confocal Fluorescence Capillary Array Scanner,”Electrophoresis17:1852-1859.
Takahashi, S. et al. (1994). “Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection,”Analytical Chemistry66(7):1021-1026.
Woolley, A.T. et al. (1994). “Ultra-High-Speed Fragment Separations Using Microfabricated Capillary Electrophoresis Chips,”PNAS USA, Biophysics91(24):11348-11352.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Multiplexed fluorescent detection in microfluidic devices does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Multiplexed fluorescent detection in microfluidic devices, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multiplexed fluorescent detection in microfluidic devices will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3410211

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.