Multiplexed detection of biological agents

Details Multiplexed detection of biological agents Multiplexed detection of biological agents

2007-11-13

2007-11-13

Martinell, James (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C536S024300, C702S019000

Reexamination Certificate

active

11123363

ABSTRACT:
Described are methods, kits and systems for multiplexed detection of biological agents in a sample, e.g., multiplexed detection of e.g., bacteria, viruses, and biological toxins. The method utilizes two markers for each agent; the presence or absence in a sample of each of the two markers per agent is determined in separate reactions; however each reaction is used to detect a single marker for multiple agents. Also disclosed is the multiplexed detection method using real time PCR. The invention provides an efficient, cost-effective, and specific method for multiplexed detection of biological agents.

REFERENCES:
patent: 6458535 (2002-10-01), Hall et al.
Zhang et al, J. Mol. Biol. 317: 225 (2002).
Molecular Biology Reagents/Protocols 1992, United States Biochemical Corporation, 1991, Cleveland, Ohio, pp. 218-219.
PCT International Search Report and Written Opinion, PCT/US05/16257, Nov. 29, 2006, 7 pages.

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