Multiplexed capillary electrophoresis system

Details Multiplexed capillary electrophoresis system Multiplexed capillary electrophoresis system

1995-05-19

1996-12-10

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204452, 204455, 204605, G01N 2726, G01N 27447

Patent

active

055827055

ABSTRACT:
The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

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