Multiplexed assay and probes for identification of HPV types

Details Multiplexed assay and probes for identification of HPV types Multiplexed assay and probes for identification of HPV types

2011-01-25

2011-01-25

Switzer, Juliet C (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C435S287200, C536S024300

Reexamination Certificate

active

07875428

ABSTRACT:
A DNA microarray, preferably in the form of a chip, contains probes which hybridize to generate primers capable of amplifying approximately 89 HPV types. These target the E1 region of the gene. The design of the chip allows for the detection of any known HPV type, based on a unique probe sequence derived from the HPV E1 region. The present assay utilizes a number of primers that can amplify from about one to six different types of HPV. A large number of primers can be used together. After amplification, the amplicons are contacted with specific probes that are unique for each HPV type. The array further employs a control sequence, which normalizes variability due to sample size.

REFERENCES:
patent: 2002/0110828 (2002-08-01), Ferea et al.
patent: 2004/0175719 (2004-09-01), Christians
patent: 2008/0287318 (2008-11-01), Kranewitter et al.
patent: WO 2005/056839 (2005-06-01), None
GenBank record having accession No. U3174 GI: 1020219, dated Oct. 17, 1995, eight pages.
Baback Gharizadeh, et al., “Viral and microbial genotyping by a combination of multiplex competitive hybridization and specific extension followed by hybridization to generic tag arrays,”Nucleic Acids Research, 2003, vol. 31, No. 22 e146.
Girish Nallur, et al., “Signal amplification by rolling circle amplification on DNA microarrays,”Nucleic Acids Research, 2001, vol. 29, No. 23 e118.
David J. Duggan, et al., “Expression profiling using cDNA microarrays,”Nature Genetics Supplement, Jan. 1999, vol. 21, 10-14.
P.E. Gravitt, et al., “Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Products by a Single-Hybridization, Reverse Line Blot Detection Method,”Journal of Clinical Microbiology, Oct. 1998, vol. 36, No. 10., 3020-3027.
Brooks E. Miner, et al., “Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR,”Nucleic Acids Research, Sep. 30, 2004, vol. 32, No. 17 e135.

Affiliated with

Also associated with

Rating

Multiplexed assay and probes for identification of HPV types does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Multiplexed assay and probes for identification of HPV types, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multiplexed assay and probes for identification of HPV types will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2647427