Multiplex spatial profiling of gene expression

Details Multiplex spatial profiling of gene expression Multiplex spatial profiling of gene expression

2005-01-07

2009-08-04

Martinell, James (Department: 1634)

Chemistry: analytical and immunological testing

Process or composition for determination of physical state...

Corrosion resistance or power

C435S007100, C530S387100, C530S350000, C530S324000, C536S023100

Reexamination Certificate

active

07569392

ABSTRACT:
The present invention provides mass tag complexes that permit simultaneously obtaining information of a plurality of biological molecules. The biological molecules may be RNA or protein, and the information includes both level of expression as well as spatial disposition within a cell or tissue. The mass tag comprise a core structure, a target binding structure (e.g., nucleic acid or peptide binding structure), a cleavable linker and a mass tag that exhibits a unique mass spectroscopy signal.

REFERENCES:
patent: 6027890 (2000-02-01), Ness et al.
patent: 2002/0042112 (2002-04-01), Koster et al.
patent: 2002/0192676 (2002-12-01), Madonna et al.
patent: WO 01/15737 (2001-03-01), None
patent: WO 02/18644 (2002-03-01), None
patent: WO 02/080649 (2002-10-01), None
patent: WO 02/097703 (2002-12-01), None
patent: WO 03/008547 (2003-01-01), None
Caprioli et al., “Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS,”Anal Chem, 69:4751-4760, 1997.
Gygi et al., “Quantitative analysis of complex protein mixtures using isotope-coded affinity tags,”Nat Biotechnol, 17:994-999, 1999.
Han et al., “Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry,”Nat Biotechnol, 19:946-951, 2001.
Koomen et al., “Mapping of surrogate markers of cellular components and structures using laser desporption/ionization mass spectrometry,”J Mass Spectrom, 35:258-264, 2000.
Onnerfjord et al., “Homogenous sample preparation for automated high throughput analysis with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry,”Rapid Commun Mass Spectrom, 13:315-322, 1999.
Rubakhin et al., “Characterization of the Aplysia californica cerebral ganglion F cluster,”J Neurophysiol, 81:1251-1260, 1999.
Stetsenko and Gait, “Efficient conjugation of peptides to oligonucleotides by ‘native ligation,’”J Org Chem, 65:4900-4908, 2000.
Todd et al., “Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization,”J Mass Spectrom, 36:355-369, 2001.
Zhou et al., “Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry,”Nat Biotechnol, 20:512-515, 2002.

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