Multiplex selection technique for identifying protein-binding si

Details Multiplex selection technique for identifying protein-binding si Multiplex selection technique for identifying protein-binding si

1997-08-06

2000-05-23

Priebe, Scott D.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435 78, 435 911, 435 912, 4353201, 435440, C12Q 168, C12P 1934, C12N 1563

Patent

active

060664525

ABSTRACT:
Methods for simultaneously selecting binding site sequences for multiple DNA-binding proteins are provided. A source of DNA-binding proteins is mixed with oligonucleotide duplexes containing a randomized internal sequence. Bound oligonucleotides are isolated, amplified and analyzed, such as by DNA sequence analysis. The oligonucleotide duplexes are also used to identify and isolate DNA-binding proteins.

REFERENCES:
patent: 5552270 (1996-09-01), Khrapko et al.
Blackwell, "Selection of protein binding sites from random nucleic acid sequences", Meth. Enzymol. 254: 604-618, 1995.
Norby et al., "Determination of recognition-sequences for DNA-binding proteins by a polymerase chain reaction assisted binding site selection method (BSS) using nitrocellulose immobilized DNA binding proteins", Nucleic Acids Res. 20: 6317-6321, 1992.
Blackwell and Weintraub, "Differences and Similarities in DNA-Binding Preferences of MyoD and E2A Protein Complexes Revealed by Binding Site Selection," Science 250: 1104-1110, 1990.
Blackwell et al., "Sequence-Specific DNA Binding by the c-Myc Protein," Science 250: 1149-1151, 1990.
Chittenden et al., "The T/E1A-Binding Domain of the Retinoblastoma Product Can Interact Selectively with a Sequence-Specific DNA-Binding Protein," Cell 65: 1073-1082, 1991.
Ellington and Szostak, "In vitro selection of RNA molecules that bind specific ligands," Nature 346: 818-822, 1990.
Funk and Wright, "Cyclic amplification and selection of targets for multicomponent complexes: Myogenin interacts with factors recognizing binding sites for basic helix-loop-helix, nuclear factor 1, myocyte-specific enhancer-binding factor 2, and COMP1 factor," Proc. Natl. Acad. Sci. USA 89: 9484-9488, 1992.
Henrissat et al., "Cellulase families revealed by hydrophobic cluster analysis," Gene 81: 83-95, 1989.
Kinzler and Vogelstein, "Whole genome PCR: application to the identification of sequence bound by gene regulatory proteins," Nucleic Acids Research 17(10): 3645-3653, 1989.
Mavrothalassitis et al., "Laboratory Methods. Defining Target Sequences of DNA-Binding Proteins by Random Selection and PCR: Determination of the GCN4 Binding Sequence Repertoire," DNA and Cell Biology 9(10): 783-788, 1990.
Pierrou et al., "Selection of High-Affinity Binding Sites for Sequence-Specific, DNA Binding Proteins from Random Sequence Oligonucleotides," Analytical Biochemistry 229: 99-105, 1995.
Pollock and Treisman, "A sensitive method for the determination of protein-DNA binding specificities," Nucleic Acids Research 18(21): 6197-6204, 1990.
Rudert and Trucco, "DNA polymers of proteins binding sequences generated by PCR,"Nucleic Acids Research 18(21): 6460, 1990.
Thiesen and Bach, "Target Detection Assay (TDA): a versatile procedure to determine DNA binding sites as demonstrated on SP1 protein," Nucleic Acids Research 18(11): 3203-3209, 1990.
Tuerk and Gold, "Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase," Science 249: 505-510, 1990.
Wright and Funk, "CASTing for multicomponent DNA-binding complexes," Trends in Biochemical Sciences 18: 77-80, 1993.

Affiliated with

Also associated with

Rating

Multiplex selection technique for identifying protein-binding si does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Multiplex selection technique for identifying protein-binding si, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multiplex selection technique for identifying protein-binding si will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1835969