Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1996-08-29
1998-04-07
Jones, W. Gary
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 536 2433, 935 17, 935 77, 935 78, C12P 1934, C12Q 170, C07H 2104
Patent
active
057363650
ABSTRACT:
Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets. In a preferred embodiment, an internal control sequence is included in the amplification reaction and coamplified with the IS6110 and 16S target sequences as an indication of sample amplification activity or to quantitate the initial amount of target sequences in the sample.
REFERENCES:
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patent: 5213961 (1993-05-01), Bunn et al.
patent: 5624825 (1997-04-01), Walker et al.
Walker et al. Nucl. Ac. Res. 20:1691-1696 (1992).
Jurgensen Stewart Russel
Nadeau James G.
Nycz Colleen M.
Schram James L.
Shank Daryl Dee
Becton Dickinson and Company
Fugit Donna R.
Jones W. Gary
Shoemaker Debra
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