Multiplex ligatable probe amplification

Details Multiplex ligatable probe amplification Multiplex ligatable probe amplification

2005-10-18

2005-10-18

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S091210, C435S006120, C536S023100, C536S024300, C536S024330

Reexamination Certificate

active

06955901

ABSTRACT:
Described is an improved multiplex ligation-dependent amplification method for detecting the presence and quantification of at least one specific single stranded target nucleic acid sequence in a sample using a plurality of probe sets of at least two probes, each of which includes a target specific region and a non-complementary region comprising a primer binding site. The probes belonging to the same set are ligated together when hybridised to the target nucleic acid sequence and amplified by a suitable primer set. By using a femtomolar amount of the probes a large number of different probe sets can be used to simultaneously detect and quantify a corresponding large number of target sequences with high specificity.

REFERENCES:
patent: 6534293 (2003-03-01), Barany et al.
patent: 6812005 (2004-11-01), Fan et al.
patent: 2003/0022182 (2003-01-01), Barany et al.
patent: WO 96/15271 (1996-05-01), None
patent: WO 97/08344 (1997-03-01), None
patent: WO 97/19193 (1997-05-01), None
patent: WO 97/45559 (1997-12-01), None
patent: WO 98/03673 (1998-01-01), None
patent: WO 98/04746 (1998-02-01), None
patent: WO 98/37230 (1998-08-01), None
Martin Wiedmann et al, “Ligase Chain Reaction (LCR)—Overview and Applications,” Cold Spring Harbor Laboratory Press, New York, Ny, vol 4, No. 4, 1994, pp. 551-564.

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