Multiple sorter monitor and control subsystem for flow...

Chemistry: analytical and immunological testing – Biological cellular material tested

Reexamination Certificate

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C209S003100, C209S003200, C209S003300, C209S004000, C209S127400, C209S552000, C209S571000, C209S576000, C209S577000, C209S578000, C209S579000, C209S906000, C250S461200, C422S073000, C422S082050, C422S082080, C422S082090, C422S082110, C436S164000, C436S172000

Reexamination Certificate

active

10819745

ABSTRACT:
An optical illumination and monitoring subsystem controls the operation of a flow cytometer having a carrier fluid that flows along a channel coupled to a droplet generator, which controls a point at which droplets break off from the carrier fluid, and a droplet sorter that is operative to cause selected droplets to be sorted along one or more droplet travel paths. The subsystem is operative to illuminate a respective droplet monitoring location along each of one or more droplet travel paths with a respective beam of light, such as that sourced from a common laser. In response to backscatter reflection from a droplet passing through the respective beam of light at a respective droplet monitoring location, the amplitude of the beam of light is increased. Then the droplet is monitored for the presence of a particle therein exhibiting detectable fluorescence as a result of the increase in the amplitude of the respective beam of light.

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