Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1993-01-15
1994-02-08
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 435284, 435285, 435291, 435310, 435311, C12Q 102, C12Q 124, C12M 300
Patent
active
052847535
ABSTRACT:
A simple chemotaxis apparatus and method wherein surface tension, capillary action, and hydrophobic forces are used to hold cell suspensions, chemotactic factors, and control fluids in place on both membrane filters and chemotaxis plates. In one embodiment, chemotactic factors and controls are placed at preselected areas on the top surface of a bottom plate, while a membrane filter topped with cell suspensions is placed above the bottom plate so that the drops of chemotactic factors and controls contact the filter membrane directly below the locations of the cell suspensions. In another embodiment, the preselected locations on the bottom plate are rimmed wells that are filled with chemotactic factors and controls. Hydrophobic coating is used to limit fluid movement on the filter membrane and on the bottom plate. Occluding materials or monolayers of cells are used to further limit the gravitational fluid flow in the preferred embodiments.
REFERENCES:
patent: 3888770 (1975-06-01), Avital et al.
patent: 3929583 (1975-12-01), Sharpe et al.
patent: 4317726 (1982-03-01), Shepel
patent: 4493815 (1985-01-01), Fernwood et al.
patent: 4514495 (1985-04-01), Schalkowsky et al.
patent: 4714674 (1987-12-01), Palladino
patent: 4912057 (1990-03-01), Guirguis et al.
patent: 5023173 (1991-06-01), Horwitz et al.
W. Falk, R. H. Goodwin, Jr. and E. J. Leonard, "A 48-Well Micro Chemotaxis Assembly for Rapid and Accurate Measurement of Leukocyte Migration," Journal of Immunological Methods, 33, pp. 239-247 (1980).
L. Harvath, W. Falk and E. J. Leonard, "Rapid Quantitation of Neutrophil Chemotaxis: Use of a Polyvinylpyrrolidone-Free Polycarbonate Membrane in a Multiwell Assembly," Journal of Immunological Methods, 37, pp. 39-45 (1980).
K. L. Richards and J. McCullough, "A Modified Mircochamber Method for Chemotaxis and Chemokinesis," Immunological Communications, 13(1), pp. 49-62 (1984).
W. L. Falk, L. Harvath and E. J. Leonard, "Only the Chemotactic Subpopulation of Human Blood Monocytes Expresses Receptors for the Chemotactic Peptide N-Formylmethionyl-Leucyl-Phenylalanine," Infection and Immunity, pp. 450-454 (May 1982).
L. Harvath and E. J. Leonard, "Two Neutrophil Populations in Human Blood with Different Chemotactic Activities: Separation and Chemoattractant Binding," Infection and Immunity, pp. 443-449 (May 1982).
"Neuro Probe 48 Well Micro Chemotaxis Chamber Manual," (1980).
Neuro Probe, Inc. Sales Brochure, "48 Well Micro Chemotaxis Chamber", 1988.
Neuro Probe, Inc. Sales Brochure, "12 Well Chemotaxis Chamber", 1988.
Neuro Probe, Inc. Sales Brochure, "12 Well Manifold Chamber", 1988.
Neuro Probe, Inc. Sales Brochure, "10 Well Chemotaxis Chamber", 1988.
Neuro Probe, Inc. Sales Brochure, "Blind Well and Boyden Chambers", 1988.
Neuro Probe, Inc. Sales Brochure, "Single Well and Four Well Separation Chambers", 1988.
Neuro Probe, Inc. Sales Brochure, "Three Tiered Chamber", 1988.
Neuro Probe, Inc. Sales Brochure, "Zigmond Chamber", 1988.
Neuro Probe, Inc. Sales Brochure, "Guirguis Chamber"; 1988.
Neuro Probe, Inc. Sales Brochure, "Accessories", 1988.
Neuro Probe, Inc.
Thornton Krisanne M.
Warden Robert J.
LandOfFree
Multiple-site chemotactic test apparatus and method does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Multiple-site chemotactic test apparatus and method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multiple-site chemotactic test apparatus and method will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-697131