Multiple selection process for binding sites of DNA-binding prot

Details Multiple selection process for binding sites of DNA-binding prot Multiple selection process for binding sites of DNA-binding prot

1996-01-24

1999-01-19

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435 911, 435 912, 536 241, C12Q 168, C07H 2104, C12P 1934

Patent

active

058612467

ABSTRACT:
Methods are provided for isolating binding sites of DNA-binding proteins. An extract, such as a nuclear extract, containing DNA-binding proteins is mixed with oligonucleotide duplexes comprising a random sequence. Bound duplexes are isolated and amplified. Methods are also provided for identifying DNA-binding proteins using isolated binding sites.

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