Multiple RNA polymerase III promoter expression constructs

Details Multiple RNA polymerase III promoter expression constructs Multiple RNA polymerase III promoter expression constructs

2011-07-26

2011-07-26

McGarry, Sean (Department: 1635)

Chemistry: molecular biology and microbiology

Vector, per se

C435S006120, C536S023100, C536S024100, C536S024500, C514S04400A

Reexamination Certificate

active

07985581

ABSTRACT:
Expression constructs comprising at least two different RNA polymerase III promoters, wherein each promoter is operably linked to a nucleic acid sequence encoding an RNA effector molecule, are disclosed herein. Further provided are expression constructs comprising multiple polymerase III promoters operably linked to sequences encoding short hairpin RNA molecules, which may comprise single and/or multiple fingers. The provided constructs are useful for in vivo delivery of RNA molecules effective in gene silencing, including of viral genes including HBV and HCV.

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