Multiple reading frame Escherichia coli expression vectors

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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4351723, 435320, 43525233, 435 70, 935 27, 935 29, 935 41, 935 48, C12P 2100, C12N 1500, C12N 120, C12N 100

Patent

active

048493511

ABSTRACT:
Vector plasmids are constructed to provide sites for insertion of a structural gene in phase with three reading frames downstream from the promoter of alkaline phosphatase gene of Escherichia coli.

REFERENCES:
patent: 4375514 (1983-03-01), Siewert et al.
Yoda et al, 1980, "Cloning of the Structural Gene of Alkaline Phosphatase of E. coli K12", Agric. Biol. Chem., v. 44(5), 1213-1214.
Kikuchi et al, 1981, "Sequence of the Signal Peptide of E. coli Alkaline Phosphatase", Agric. Biol. Chem., v. 45(10), 2401-2402.
Kikuchi et al, 1981, "The Nucleotide Sequence of the Promoter and the Amino-Terminal Region of Alkaline Phosphatase Structural Gene . . . ", Nucleic Acids Res., v. 9, 5671-5678.

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