Multiple oxidative indicator system for visual determination of

Details Multiple oxidative indicator system for visual determination of Multiple oxidative indicator system for visual determination of

1987-09-11

1989-08-08

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 14, 435 25, 435174, 435192, 435805, C21Q 128, C21Q 154, C21Q 126, C12N 1100

Patent

active

048552283

ABSTRACT:
The invention involves visual determination of hydrogen peroxide with a multiple oxidative indicator system capable of generating different hues at different concentrations of hydrogen peroxide or at different concentrations of an analyte from which hydrogen peroxide is generated by means of an analyte specific oxidase. A first indicator component of the multiple oxidative indicator system is oxidized from a particular hue to a different hue which is preferably essentially colorless. A second indicator component is oxidized to a hue which is visually distinct from the first indicator component in either of its hues. A third indicator component may also be present which is oxidized to provide a colorimetric response. Each component is oxidized only by hydrogen peroxide and peroxidase. The multiple oxidative indicator system is capable of generating visually distinct hues at different concentrations of hydrogen peroxide and can be used to generate a "rainbow" of hues when the indicator components are oxidized sequentially.

REFERENCES:
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patent: 2981606 (1961-04-01), Keston
patent: 3008879 (1961-11-01), Harvill
patent: 4042329 (1977-08-01), Hochstrasser
patent: 4132527 (1979-01-01), Maekawa et al.
patent: 4234313 (1980-11-01), Faulkner
patent: 4716110 (1987-12-01), Wada et al.

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