Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1997-09-10
2000-02-01
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435174, 435176, 435178, 435182, 530402, 530810, 530811, 530813, 530815, 530817, C12N 1100, C12N 1108, C07K 1700, C07K 1708
Patent
active
060201754
ABSTRACT:
Multiple layered functional thin films fixed on a solid support are provided which comprise multiple layers of functional molecules (such as enzymes and other proteins, pigments and dyes) admixed with polymer ions in combination with multiple layers of polymer ions without the functional molecules. The films are prepared by immersing a solid support having an electric charge in an admixed polymer ion-functional molecule solution having a net electric charge opposite to that of the solid support followed by immersing the solid support in a polymer ion solution having a net electric charge opposite to that of the admixed polymer ion-functional molecule solution, and repeating at least once the immersings of the solid support in the solutions. Alternatively, the films are prepared by immersing the solid support in a polymer ion solution having a net electric charge opposite to that of the solid support followed by immersing the solid support in an admixed polymer ion-functional molecule solution having a net electric charge opposite to that of the polymer ion solution, and repeating at least once the immersings of the solid support in the solutions.
REFERENCES:
Biotechnology and Bioengineering article entitled "Sequential Actions of Glucose Oxidase and Peroxidase in Molecular Films Assembled by Layer-by-Layer Alternate Adsorption" vol. 51, pp. 163-167 (1996).
J.Am. Chem. Soc., vol. 117, No. 22, pp. 6117-6123 (1995) article entitled "Assembly of Multicomponent Protein Films by Means of Electrostatic Layer-by-Layer Adsorption".
Ariga Katsuhiko
Kunitake Toyoki
Lvov Yuri
Onda Mitsuhiko
Japan Science and Technology Corporation
Naff David M.
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