Multiple-component binding assay system and method of making and

Details Multiple-component binding assay system and method of making and Multiple-component binding assay system and method of making and

1981-10-05

1984-07-10

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving iga, igd, ige, or igm

422 61, 422 68, 422 71, 436530, 436804, 436808, 436810, G01N 3358, G01N 3360, G01N 2306, B65D 7100

Patent

active

044593600

ABSTRACT:
A multiple component binding assay system, and related methods of making and using it, having a plurality of coated filaments mounted at their opposite ends on a support, in a predetermined spaced relationship, for simultaneously screening a liquid test sample for a plurality of components. The system is particularly suitable for screening the sample for IgE class antibodies specific for certain allergens. The filaments are preferably cotton threads, each thread binding a different allergen through a covalent bond. The support, which is preferably cut away adjacent the mid-portion of each filament to reduce background interference, is composed of a plastic or other water insoluble material, permitting inexpensive and relatively simple manufacture. After any IgE class antibodies present in the liquid sample have reacted with the corresponding allergens coated on the filaments, they are detected using second antibodies labeled with radioactive, fluorescent or enzymatic tracers. By using a high energy radioactive tracer, such as .sup.125 I, autoradiographic techniques with phosphor screen enhancement allow for diagnostic analysis by a densitometer or even the unaided human eye.

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