Multiple chromogen enzyme targeting (MCET) for use in bacterial

Details Multiple chromogen enzyme targeting (MCET) for use in bacterial Multiple chromogen enzyme targeting (MCET) for use in bacterial

1994-06-21

1996-04-23

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 42, 435 34, 435 29, 435 38, 435 39, 435 14, 435 4, 435849, C12Q 134, C12Q 104, C12P 3900, C12N 100

Patent

active

055102433

ABSTRACT:
The invention is directed to a process and a medium for simultaneous determination of the number and presence of living fecal coliform and Escherichia coli in a sample comprising 6-O-alpha-D-galactopyranosyl-D-glucose or isopropyl-beta-D-thiogalactoside as a galactosidase inducer and methyl-beta-D-glucuronide as a glucuronidase inducer. The sterile semi-solid medium also comprises non-target bacterial inhibitors, target bacterial enhancers, and multiple fluorogen and/or chromogan substrates that produce color and fluorescence upon cleavage by a specific enzyme expressed by the target bacteria in which expression is enhanced. The simultaneous detection of total coliforms via its expression of beta-galactosidase, and Escherichia coli as the target bacteria via its expression of beta-galactosidase and beta-glucuronidase is achieved rapidly and efficiently using this medium.

REFERENCES:
patent: 3095343 (1960-09-01), Berger
patent: 3111702 (1961-07-01), Berger
patent: 4263967 (1981-04-01), McNab et al.
patent: 4598046 (1986-01-01), Kimura et al.
patent: 4622297 (1986-11-01), Kappner et al.
patent: 4684609 (1987-08-01), Hsu
patent: 4806313 (1989-02-01), Ebersole et al.
patent: 4829969 (1989-05-01), Ray
patent: 4868110 (1989-09-01), Des Rosier et al.
patent: 4923804 (1990-05-01), Ley et al.
patent: 4925789 (1990-05-01), Edberg
patent: 4933291 (1990-06-01), Daiss et al.
patent: 5006462 (1991-04-01), Gattaz
patent: 5171537 (1992-12-01), Wainwright et al.
patent: 5210022 (1993-05-01), Roth et al.
patent: 5223402 (1993-06-01), Abbas et al.
patent: 5358854 (1994-10-01), Ferguson
Nucleic Acids Research, vol. 13 No. 15, 1985 pp. 5457-5469.
Brenner et al, Applied & Environmental Microbiology, pp. 3534-3544, Nov. 1993.
Mamas et al, (Chem Abstract) 91: 68210 (1979).

Affiliated with

Also associated with

Rating

Multiple chromogen enzyme targeting (MCET) for use in bacterial does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Multiple chromogen enzyme targeting (MCET) for use in bacterial , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multiple chromogen enzyme targeting (MCET) for use in bacterial will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2307685