Multiple capillary biochemical analyzer with barrier member

Details Multiple capillary biochemical analyzer with barrier member Multiple capillary biochemical analyzer with barrier member

1996-01-30

1996-10-22

Gorgos, Kathryn

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204452, G01N 2726, G01N 27447

Patent

active

055672949

ABSTRACT:
A multiple capillary biochemical analyzer for sequencing DNA and performing other analyses, in which a set of capillaries extends from wells in a microtiter plate into a cuvette. In the cuvette the capillaries are held on fixed closely spaced centers by passing through a sandwich construction having a pair of metal shims which squeeze between them a rubber gasket, forming a leak proof seal for an interior chamber in which the capillary ends are positioned. Sheath fluid enters the chamber and entrains filament sample streams from the capillaries. The filament sample streams, and sheath fluid, flow through aligned holes in a barrier member spaced close to the capillary ends, into a collection chamber having a lower glass window. The filament streams are illuminated above the barrier member by a laser, causing them to fluoresce. The fluorescence is viewed end-on by a CCD camera chip located below the glass window. The arrangement ensures an equal optical path length from all fluorescing spots to the CCD chip and also blocks scattered fluorescence illumination, providing more uniform results and an improved signal to noise ratio.

REFERENCES:
patent: 5274240 (1993-12-01), Matties et al.
patent: 5324401 (1994-06-01), Yeung et al.
patent: 5366608 (1994-11-01), Kambara
patent: 5439578 (1995-08-01), Dovichi et al.
patent: 5468364 (1995-11-01), Fuji

Affiliated with

Also associated with

Rating

Multiple capillary biochemical analyzer with barrier member does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Multiple capillary biochemical analyzer with barrier member, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multiple capillary biochemical analyzer with barrier member will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2355762