Multiparameter FACS assays to detect alterations in exocytosis

Details Multiparameter FACS assays to detect alterations in exocytosis Multiparameter FACS assays to detect alterations in exocytosis

2005-05-24

2005-05-24

Le, Long V. (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C424S001410, C424S406000, C435S007200, C436S164000, C436S172000, C356S485000, C356S492000, C356S495000

Reexamination Certificate

active

06897031

ABSTRACT:
Described is a method for screening for alterations in exocytosis of a population of cells. The cells are sorted by a FACS machine by assaying for alterations in at least three of the properties selected from the group consisting of light scattering, fluorescent dye uptake, fluorescent dye release, annexin granule binding, surface granule enzyme activity, and the quantity of granule specific proteins. Methods for screening for bioactive agents capable of modulating exocytosis in a cell are also described. The methods provide for reduced background and increased specificity without increasing the time or steps involved in assaying for exocytosis.

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