Multimerized highly functional antifreeze protein

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence

Reexamination Certificate

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C530S333000, C530S300000

Reexamination Certificate

active

07041788

ABSTRACT:
The present invention provides an antifreeze protein, wherein thermal hysteresis activity and ice recrystallization inhibitory activity are artificially improved. The antifreeze protein is multimerized using a polypeptide linker to improve the thermal hysteresis and ice nuclei growth inhibitory effects in low concentrations.

REFERENCES:
Christiansen et al. “The role of the MoFe protein alpha-125-Phe and beta-235-Phe residues in Azotobacter vinelandii MoFe protein Fe protein interaction”. Journal of Inorganic Biochemistry. 2000. vol. 80, pp. 195-204.
Sorlie et al. “Mechanistic features and structure of the nitrogenase alpha-Gin-195 MoFe protein”. Biochemistry. 2001. vol. 40, pp. 1540-1549.
USPOT sequence alignments for Wang et al. protein versus SEQ ID Nos: 2 and 4.
Miura Kazunori et al: “NMR analysis of type III antifreeze protein intramolecular dimer: Structural basis for enhanced acativity”, Journal of Biological Chemistry, vol. 276, No. 2, Jan. 12, 2001, pp. 1304-1310, XP002236044.
Wang Xin et al.: “Antifreeze peptide heterogeneity in an antarctic eel pout includes an unusually large major variant comprised of two 7 kDa type III AFPs linked in tandem.”, Biochimica Et Biophysica Acta, vol. 1247, 'No. 2, 1995, pp. 163-172, XP002236045.
Wang Xin et al.: “Genomic basis for antifreeze peptide heterogeneity and abundance in an Antarctic eel pout: Gene structures and organization,” Molecular Marine Biology and Biotechnology, vol. 4, No. 2, 1995, pp. 135-147, XP009007178.
Miura Kazunori et al.: “Determination of the solution structure of the N-domain plus linker of antarctic eel pout antifreeze protein RD3,” Journal of Biochemistry (Tokyo), vol. 126, No. 2, Aug. 1999, pp. 387-394, XP002236046.
Graether Steffen P et al: “Quantitative and analysis of type III antifreeze protein structure and function,” Journal of Biological Chemistry, vol. 274, No. 17, Apr. 23, 1999, pp. 11842-11847, XP002236047.
Ewart K V et al: “Structure, function and evolution of antifreeze proteins,” CMLS Cellular and Molecular Life Sciences, vol. 55, No. 2, Feb. 1999, pp. 271-283, XP002236048.

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