Multilayer enzyme electrode membranes and methods of making same

Details Multilayer enzyme electrode membranes and methods of making same Multilayer enzyme electrode membranes and methods of making same

1996-07-12

1997-12-09

Williams, Hezron E.

Measuring and testing

Liquid analysis or analysis of the suspension of solids in a...

73 6141, 73 6171, 73 6447, 422 88, 435288, 435817, 435 14, 204414, 204403, G01N 2726, G01N 33535, G01N 3366, H01L 2978

Patent

active

056963149

ABSTRACT:
An electrochemical sensor is designed for use in detecting a concentration of an analyte in a sample solution. The sensor to have a longer useful lifetime, improved stability and improved response time for single or multiple use applications. In one aspect, some of these goals are achieved by providing the multilayer enzyme electrode membrane having both nonporous and microporous layers. The microporous layer is formed from a polymer binder, a polymer powder, a surfactant and a mineral powder. In another aspect, certain of these goals are achieved by the use of a layer comprised of an enzyme disposed within a polymer matrix. In certain embodiments, the multilayer enzyme electrode membrane includes an outermost layer which is capable of dissolving in the sample solution such that the interface between the multilayer enzyme electrode membrane and the sample solution is continually renewed. The sensor provides an electrical current output proportional to the reagent chemical interaction of the analyte with an immobilized enzyme layer during exposure of the sensor in such solution.

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