Multilayer enzyme electrode membrane

Details Multilayer enzyme electrode membrane Multilayer enzyme electrode membrane

1981-11-05

1983-11-29

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

204403, 435 14, 435180, 435182, 435288, C12N 1112, C12N 1108, C12N 1104, C12M 140

Patent

active

044181485

ABSTRACT:
A contiguous multilayer membrane for use with an electrochemical sensor is prepared comprising a first relatively nonporous dense polymer layer, a second polymer layer less dense and more porous than the first layer, a third layer containing an enzyme and a fourth polymer layer less dense and more porous than the first layer. The polymer is preferably cellulose acetate and the second and fourth layers are prepared with a solvent and nonsolvent for the polymer. The membrane may contain multiple enzyme layers separated by a polymer layer. The multilayer membrane provides advantages of higher substrate conversion, homogeneous distribution of enzyme and/or minimized interference with analyte diffusion.

REFERENCES:
patent: 3715277 (1973-02-01), Dinelli et al.
patent: 3947325 (1976-03-01), Dinelli et al.
patent: 3979274 (1976-09-01), Newman
patent: 4004980 (1977-01-01), Emery et al.
patent: 4090022 (1978-05-01), Tsao et al.
patent: 4092233 (1978-05-01), Clemens et al.
Kolarik et al., Glucose Isomerase Cells Entrapped in Cellulose Acetates, Immobilized Enzymes in Food and Microbial Processes, Plenam Press, N.Y., 1974 (pp. 71-83).

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