Multilayer element for analysis

Chemistry: analytical and immunological testing – Optical result – With reagent in absorbent or bibulous substrate

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422 56, 435805, 436810, G01N 3122, G01N 3352, G01N 3354, G01N 3358

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active

044593589

ABSTRACT:
A multilayer element for the analysis of a liquid containing an analyte comprising a reaction layer which retains a predetermined amount of a diffusible reagent containing a detectable species; said reagent adapted to be rendered substantially non-diffusible by said analyte as a function of the concentration of said analyte; a registration layer adapted to receive reagent diffusing thereto; means for detecting said reagent in said registration layer and an insulating layer intermediate said reaction layer adapted to provide discrimination between detectable species in said reagent layer and said registration layer.

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patent: 4258001 (1981-03-01), Pierce et al.
patent: 4292272 (1981-09-01), Kitajima et al.
Ouchterlony et al., Immunochemistry, Third ed. Blackwell Scientific Publication, Oxford, England, vol. 1 Chapter 19.

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