Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1979-06-21
1981-11-17
Padgett, Benjamin R.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 210264, 210284, 422 61, 424 8, 424 12, G01N 3348, B01N 2310, B65D 700
Patent
active
043011390
ABSTRACT:
A specific binding assay method, and a test device and test kit for use therein, for determining a liqand, such as an antigen or antibody, in, or the ligand binding capacity of, a liquid medium, particularly a body fluid such as serum, wherein the unknown ligand competes with a labeled component, such as a radiolabeled form of the ligand or of a binding analog of the ligand, for binding with a binding partner, and wherein separation of the resulting bound-species and free-species of the labeled component is accomplished by allowing the liquid reaction mixture to be drawn by capillary action into a column comprising a bed of an adsorbent material selective for one of the two species. The improvement comprises using a column containing at least one additional bed of capillarily absorbent material disposed above said adsorbent bed and which is substantially nonadsorbent relative to the one of said two species which said adsorbent bed selectively binds. The use of specific types of capillarily absorbent beds in addition to the selectively adsorbent bed to form multilayer columns overcomes several unforeseen problems encountered in using the previously known column chromatography method for certain assay applications.
REFERENCES:
patent: 4094647 (1978-06-01), Deutsch et al.
patent: 4168146 (1979-09-01), Grubb et al.
patent: 4189304 (1980-02-01), Adams, Jr. et al.
patent: 4205058 (1980-05-01), Wagner et al.
Feingers Judith
Pick Anthony J.
Wagner Daniel B.
Ames-Yissum Ltd.
Klawitter Andrew L.
Nucker Christine M.
Padgett Benjamin R.
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