Multilayer analytical element for salicylate assay

Details Multilayer analytical element for salicylate assay Multilayer analytical element for salicylate assay

1994-03-25

1995-12-12

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 26, 435 28, 435188, 435805, 422 56, 422 57, C12Q 126, C12N 996, G01N 2100

Patent

active

054749077

ABSTRACT:
A multilayer analytical element for measuring salicylate is disclosed. The element has a support. The support is coated with the following layers, top down to the support:
a) a spreading layer,
b) a dye layer having a pH greater than 6.5 and comprising tyrosinase and a water-insoluble hydrazone coupling agent,
c) a barrier layer that prevents passage of molecules having a molecular weight in excess of 5,000, and
d) a reagent layer comprising salicylate hydroxylase and nicotinamide adenine dinucleotide (NADH), wherein the spreading layer comprises valproic acid, 8-anilino-1-naphthalenesutfonic acid (ANS) or the magnesium salt of 8-anilino-1-naphthalenesulfonic acid.

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Bertocchi P., A Flow-Through System for the Determination . . . Clin Chim Acta 207 (1992) pp. 205-213.
Ferry D., Binding of Valproic Acid to Protein Chem Abstracts 91: 68277e 1978.

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