Multilayer analytical element containing primary amine buffer an

Details Multilayer analytical element containing primary amine buffer an Multilayer analytical element containing primary amine buffer an

1993-01-19

1995-05-16

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 25, 435 14, 435 4, 422 56, 422 60, 422 681, 436132, 436131, 436170, 436164, C12Q 126, G01N 2100, G01N 3300, G01N 2177

Patent

active

054160049

ABSTRACT:
A multilayer analytical element has been prepared for accurate and rapid colorimetric determination of ethanol in aqueous specimens using alcohol dehydogenase and an oxidized nicotinamide coenzyme. The element includes three reagent layers beneath a porous spreading layer. The middle reagent layer has a crosslinked hydrophilic binder, while the other two reagent layers have uncrosslinked binders. In addition, both uncrosslinked reagent layers include a relatively high amount of a buffer having a primary amine, which buffer maintains the layer pH at from about 8 to about 10. Alcohol dehydrogenase is in the reagent layer next to the porous spreading layer.

REFERENCES:
patent: 3493467 (1970-02-01), Drell et al.
patent: 3926736 (1975-12-01), Bucolo
patent: 3941659 (1976-03-01), Koch et al.
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4042335 (1977-08-01), Clement
patent: 4132528 (1979-01-01), Eikenberry et al.
patent: 4144305 (1979-03-01), Cottrell, Jr. et al.
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4292272 (1981-09-01), Kitajima et al.
patent: 4430436 (1984-02-01), Koyama et al.
patent: 4490465 (1984-12-01), Limbach et al.
patent: 4781890 (1988-11-01), Arai et al.
patent: 4810633 (1989-03-01), Bauer et al.
patent: 4900665 (1990-02-01), Terashima et al.
patent: 4937047 (1990-06-01), Kobayashi et al.
patent: 5023052 (1991-06-01), Nagatomo et al.
patent: 5028528 (1991-07-01), Frickey et al.
patent: 5047322 (1991-09-01), Emmons et al.
patent: 5118472 (1992-06-01), Tanaka et al.

Affiliated with

Also associated with

Rating

Multilayer analytical element containing primary amine buffer an does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Multilayer analytical element containing primary amine buffer an, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multilayer analytical element containing primary amine buffer an will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-637212