Multilayer analytical element containing crosslinked binder and

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435182, 436106, 436132, 422 57, 422 60, C12Q 132, G01N 3300

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active

054299314

ABSTRACT:
A multilayer analytical element has been prepared for accurate and rapid colorimetric determination of ethanol in aqueous specimens using alcohol dehydrogenase and an oxidized nicotinamide coenzyme. The element includes at least one reagent layer beneath a porous spreading layer. This reagent layer has a crosslinked polymeric binder prepared, in part, with a polymerizable monomer having a halomethylcarbonyl, haloethylcarbonyl, halomethylsulfonyl or haloethylsulfonyl group. Alcohol dehydrogenase is in a layer of the element for reaction with the analyte.

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Sigma Catalog 1993 Diagnostic Reagents, under the heading of Alcohol (Ethanol).

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