Multilayer analysis film for analyzing transaminase

Details Multilayer analysis film for analyzing transaminase Multilayer analysis film for analyzing transaminase

1985-09-09

1990-01-30

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 25, 435 28, 435805, 422 56, 422 57, C12Q 152, C12Q 126, C12Q 128

Patent

active

048973475

ABSTRACT:
A multilayer analysis film for assaying transaminase in a coupled reaction system by a kinetic method comprises (1) a porous layer containing a transaminase substrate, (2) a coloring indicator layer for the detection of hydrogen peroxide, and (3) a transparent support, integrally laminated in this order. Pyruvate oxidase is located in a large excess in the uppermost porous layer where a rate-determining step receives maximum exposure to oxygen in the air. By the use of the analysis film, transaminase activity can be assayed with high sensitivity.

REFERENCES:
patent: 4166763 (1979-09-01), Esders et al.
patent: 4246342 (1981-01-01), Misaki et al.
patent: 4292272 (1981-09-01), Kitajima et al.
patent: 4452887 (1984-06-01), Kitajima et al.
patent: 4503145 (1985-03-01), Katsuyama et al.

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