Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1997-06-17
2000-10-31
Housel, James C.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
422 681, 4352871, G01N 33543
Patent
active
061401358
DESCRIPTION:
BRIEF SUMMARY
The present invention relates to arrays of selected immobilized molecules for interaction analysis and processes for the production thereof. More precisely the invention relates to arrays in which each molecule has a predetermined, identifiable position in the array. The array is obtainable by bundling and fixing together flat or elongated, thin carrier elements, each element having immobilized thereto a selected molecule and finally sectioning the bundles.
Two-dimensional arrays of molecules will become an important type of devices in many analytical approaches. The general benefit of such devices is the potential for rapid, simultaneous analysis with respect to large numbers of entities. It is already known to use arrays of molecules, e.g. biomolecules such as oligonucleotides, peptides etc. in devices for interaction analysis. According to WO 89/10977, an array of the whole or a chosen part of a complete set of oligonucleotides is attached to a surface, which oligonucleotides are capable of taking part in hybridization reactions. In this document as well as in others it is suggested to produce the array by some sort of printing device. An array of the oligonucleotides could be laid down on a surface by using a pen plotter, a laser typesetter or an ink jet printer for example. Another method to construct large arrays of e.g. peptides is suggested which combines solid phase organic chemistry, photo-labile protecting groups and photolitography (Fodor, S. P. A.,Read, J. L., Pirrung, M. C., Stryer, L., Lu, A. T. and Solas D. (1991) Science 251, 76-773). A surface is derivatized with amine linkers which are blocked by photochemically cleavable protecting groups. The surface is selectively irradiated with light to liberate free amines, which can be coupled to photochemically blocked building blocks. The process is repeated with different regions of the synthesis surface being exposed to light, until a desired array of compounds is prepared. The pattern of photolysis and the order of addition of building blocks define the products and their locations. In other methods with solid phase chemistry oligonucleotide arrays were built by directing the base additions to channels created by barrier between plates; alternate bases are added through channels placed at right angles to the previous addition and finer spacing is used as the length of the oligonucleotides and the complexity of the set increases. (Nucleic Acids Research, 1993, Vol. 21, No. 9 2267-2268). These methods are however tedious and time consuming where each array has to be produced individually dot by dot using printing devices or synthesizing each dot individually on site. Another draw back with the generating of the required compound on site is that it is not possible to check the correctness of the obtained molecule before it is immobilized.
The object of the present invention is to provide improved methods for the production of arrays of molecules.
A further object of the present invention is to offer improved arrays of immobilized molecules and especially arrays obtainable by the methods according to the invention.
These objects are achieved by the array and the methods for the production thereof as claimed in the claims. According to the invention a layer of a two dimensional array of interconnected and regularly ordered carrier elements is obtained, each individual element having immobilized thereto a selected molecule and having an identifiable position in the array.
According to one aspect of the invention, there is obtained an array of selected immobilized molecules for interaction analysis in which each molecule has a predetermined, identifiable position in the array. The array is obtainable by a regular way, each element having immobilized thereto a selected molecule and having an identifiable position in the array
According to a further aspect the invention provides a method for the production of an array of selected immobilized molecules for interaction analysis in which each molecule has a predetermined, identifiable position in the arr
REFERENCES:
patent: 4116638 (1978-09-01), Kenoff
patent: 4225575 (1980-09-01), Piasio et al.
patent: 4558013 (1985-12-01), Marinkovich et al.
patent: 4567149 (1986-01-01), Sell et al.
patent: 4657869 (1987-04-01), Richards et al.
patent: 4708931 (1987-11-01), Christian
patent: 4743541 (1988-05-01), Higgins
patent: 4981653 (1991-01-01), Marino
patent: 5063081 (1991-11-01), Cozzette et al.
patent: 5082768 (1992-01-01), Burd et al.
patent: 5215716 (1993-06-01), Arai
patent: 5324401 (1994-06-01), Yeung et al.
patent: 5405783 (1995-04-01), Pirrung et al.
patent: 5424186 (1995-06-01), Fodor et al.
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5451683 (1995-09-01), Barrett et al.
patent: 5498324 (1996-03-01), Yeung et al.
patent: 5516644 (1996-05-01), Yamauchi et al.
patent: 5552272 (1996-09-01), Bogart et al.
patent: 5552322 (1996-09-01), Nemoto et al.
patent: 5556529 (1996-09-01), Nemoto
patent: 5605662 (1997-02-01), Heller et al.
patent: 5632957 (1997-05-01), Heller et al.
patent: 5648213 (1997-07-01), Reddy
patent: 5763263 (1998-06-01), Dehlinger
patent: 5804384 (1998-09-01), Muller et al.
patent: 5812272 (1998-06-01), King et al.
patent: 5814524 (1998-09-01), Walt et al.
patent: 5843767 (1998-12-01), Beattie
"A Novel Method for the Analysis of Multiple Sequence Variants by Hybridisation to Oligonucleotides"--Uwe Maskos and Edwin M. Southern Dept. of Biochemistry, University of Oxford, Nucleic Acids Research, 1993, vol. 21, No. 9, 2267-2268.
"Single-Base Mutational Analysis of Cancer and Genetic Diseases Using Membrane Bound Modified Oligonucleotides"--Yong Zhang, M.Y. Coyne, S.G. Will, C.H. Levenson, E.S. Kawasaki; Dept. of Human Genetics and Nucleic Acid Chemistry, Nucleic Acids Research, vol. 19, No. 14, 3929-3933, 1991.
"Light-Directed, Spatially Addressable Parallel Chemical Synthesis"--Stephen P.A. Fodor, J.L. Read, M.C. Pirrung, L. Stryner, A.T. Lu, D. Solas; Affymax Research Institute; Science, vol, 251; Feb. 15, 1991; 767-773.
Khorlin Alexander
Landegren Ulf
Mendel-Hartvig Maritha
Ohman Ove
Housel James C.
Portner Ginny Allen
LandOfFree
Multifunctional surfaces does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Multifunctional surfaces, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multifunctional surfaces will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2050237