Multifunctional single chain glycoprotein hormones...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.

Reexamination Certificate

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Details

C530S398000, C435S069400, C435S069700, C424S192100, C424S198100

Reexamination Certificate

active

06987172

ABSTRACT:
Glycosylated or nonglycosylated molecules of the formulain-line-formulae description="In-line Formulae" end="lead"?β1-(linker1)n1-β2-(linker2)n2-β3-(linker3)n3-α;  (1)in-line-formulae description="In-line Formulae" end="tail"?in-line-formulae description="In-line Formulae" end="lead"?β1-(linker1)n1-β2-(linker2)n2-α-(linker3)n3-β3;  (2)in-line-formulae description="In-line Formulae" end="tail"?in-line-formulae description="In-line Formulae" end="lead"?β1-(linker1)n1-α-(linker2)n2-β2-(linker3)n3-β3;  (3)in-line-formulae description="In-line Formulae" end="tail"?andin-line-formulae description="In-line Formulae" end="lead"?α-(linker1)n1-β1-(linker2)n2-β2-(linker3)n3-β3  (4)in-line-formulae description="In-line Formulae" end="tail"?wherein α is the α subunit of a vertebrate glycoprotein hormone or a variant thereof;each β is independently a glycoprotein β subunit or a variant thereof,each “linker” is a hydrophilic, flexible spacer equivalent to a peptide containing 1–100 amino acid residues; andeach n is a 0 or 1;said compound optionally comprising one or more additional βx(linkerx)nxand/or one or more additional α subunits are useful in protocols to enhance fertility in humans and in animals.

REFERENCES:
patent: 5705478 (1998-01-01), Boime
patent: 5958737 (1999-09-01), Boime et al.
patent: 6103501 (2000-08-01), Boime et al.
patent: 6225449 (2001-05-01), Boime
patent: 6242580 (2001-06-01), Boime et al.
patent: 6635256 (2003-10-01), Boime et al.
patent: 6689365 (2004-02-01), Boime
patent: 6693074 (2004-02-01), Boime et al.
patent: WO 90/09800 (1990-09-01), None
patent: WO 91/16922 (1991-11-01), None
patent: WO 94/24148 (1994-10-01), None
patent: WO 95/22340 (1995-08-01), None
patent: WO 96/05224 (1996-02-01), None
patent: WO 99/25849 (1999-05-01), None
patent: WO 00/23473 (2000-04-01), None
Narayan et al. “A Biologically Active Single Chain Human Chorionic Gonadotropin Analog with Altered Receptor Binding Properties” Endocrinology 140:57-71 (1999).

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