Multifunctional RNA having self-processing activity, the prepara

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4352404, 4352523, 514 44, 536 231, 536 241, 935 33, 935 34, 935 44, C12N 1500, C12N 500, A01N 4304, C07H 2102

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active

057078408

ABSTRACT:
A multifunctional RNA having self-processing activity, the preparation thereof and the use thereof.
Host cells can be transformed so that they express ribozyme RNA and antisense RNA which are connected with each other via a spacer. The RNA molecules can, for example, be complementary to a certain viral RNA. Plants which have been transformed with genes coding for RNA of this type show a significantly improved resistance to viruses.

REFERENCES:
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Chuat & Galibert, Can Ribozymes Be Used to Regulate Procaryote Gene Expression?, Biochemical and Biophysical Research Communications 162(3) :1025-1029 (Aug. 15, 1989).
Matt Cotten, The in vivo application of ribozymes, Tibtech 8:174-178 (Jul. 1990).
Rossi and Sarver, RNA enzymes (ribozymes) as antiviral therapeutic agents, Tibtech, 8:179-183 (Jul. 1990).
Chang et al., Ribozyme-Mediated Site-Specific Cleavage of the HIV-1 Genome, Clinical Biotechnology, 2(1):23-31 (Jan./Feb. 1990).
Evans et al., The effects of ribozymes on gene expression in plants, Biochemical Society Transactions 20:344S (1992).
Rezaian et al., Plant Molecular Biology 11:463-471 (1988).
Cuozzo et al., Biotechnology 6(5):549-557 (May 1988).
Forster et al. (1988), Nature vol. 334, pp. 265-267.
Walbot et al. (1988), Nature, vol. 334, pp. 196-197.

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