Stock material or miscellaneous articles – Coated or structually defined flake – particle – cell – strand,... – Particulate matter
Patent
1989-08-28
1992-08-25
Brown, Johnnie R.
Stock material or miscellaneous articles
Coated or structually defined flake, particle, cell, strand,...
Particulate matter
560155, 536 27, 536 28, 536 29, 501 33, C07H 100, C07H 2104, C03C 1200, C07C21102
Patent
active
051418134
ABSTRACT:
The subject invention concerns a novel multifunctional solid support reagent which is useful in solid phase oligonucleotide synthesis. Specifically, the reagent is used in a solid phase oligonucleotide process to chemically modify the 3' terminus of a synthetic oligonucleotide with any chemical functional group. The invention reagent can be used to construct 3' labeled oligonucleotide hybridization probes to detect the presence of a target polynucleotide in biological and clinical samples.
REFERENCES:
Saili, R. K., S. Scharf, F. Faloona, K. B. Mullis, G. R. Horn, H. A. Erlich, and N. Arnheim (1985) "Enzymatic Amplification of .beta.-glogin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia", Science 230: 1350-1354.
Embury, S. H., S. J. Scharf, R. K. Saiki, M. A. Gholson, M. Golbus, N. Arnheim, and H. A. Erlich (1987) "Rapid prenatal diagnosis of sickle cell anemia by a new method of DNA analysis," N. Engl. J. Med. 316:656-660.
Liu, E., B. Hjelle, R. Morgan, F. Hecht, and J. M. Bishop (1987) "Mutations of the Kirsten- ras proto-oncogene in human preleukaemia," Nature 330:186-188.
Rodenhuis, S., M. L. van de Wetering, W. J. Mooi, S. G. Evers, N. van Zandwijk and J. L. Bos (1987) "Mutational activity of the K-ras oncogene," N. Engl. J. Med. 317:929-935.
Agrawal, S., C. Christondoulou, and M. J. Gait (1986) "Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic ologodeozyribonucleotides," Nucl. Acids Res. 14:6227-6245.
Connolly, B. A. (1987) "The synthesis of oligonucleotides containing a primary amino group at the 5'-terminus," Nucl. Acid Res.15:3131-3139.
Jablonski, E., E. W. Moomaw, R. H. Tullis, and J. L. Ruth (1986) "Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes," Nucl. Acids Res. 14:6115-6128.
Haralambidis, J., M. Chai, and G. W. Tregear (1987) "Preparation of base-modified nucleosides suitable for non-radioactive label attachment and their incorporation into synthetic oligodeoxyribonucleotides," Nucl. Acids Res. 15:4857-4876.
Li, P., P. P. Medon, D. C. Skingle, J. A. Lanser, and R. H. Symons (1987) "Enzyme-linked synthetic oligonucleotide probes: non-radioactive detection of enterotoxigenic Echerichia coli in faecal specimens," Nucl. Acid Res. 15:5275-5287.
Smith, L. M., S. Fung, M. W. Hunkapiller, T. J. Hunkapiller, L. E. Hood (1985) "The Synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis," Nucl. Acids Res. 13:2399-2412.
Sproat, B. S., B. Beijer, and P. Rider (1987) "The synthesis of protected 5'-mercapto-2', 5'-dideoxyribonucleoside-3'-O-phosphoramidites; uses of 5'-mercapto-oligodeoxyribonucleotides," Nucl. Acids Res. 15:4837-4848.
Ansorge, W., B. Sproat, J. Stegemann, C. Schwager, and M. Zenke (1987) "Automated DNA sequencing: ultrasensitive detection of fluorecent bands during electrophoresis," Nucl. Acids Res. 15:4593-4602.
Sproat, B. S., B. Beijer, and P. Rider (1987) "The synthesis of protected 5'-dideoxyribonucleoside-3'-O-phosphoramidites; application of 5'-aminooligodeoxyribonucleotides," Nucl. Acids. Res. 15:6181-6196.
Sinha, N. D. and R. M. Cook (1988) "The preparation and application of functionalised synthetic oligonucleotides: III. Use of H-phosphonate derivatives of protected amino-hexanol and mercapto-propanol or -hexanol," Nucl. Acids Res. 16:2659-2669.
Lemaitre, M., B. Bayard, and B. Lebleu (1987) "Specific antiviral activity of a poly(L-lysine)-conjugated oligodeoxyribonucleotide sequences complimentary to vesicular somatitis virus N protein mRNA initiation site," Proc. Natl. Acad. Sci. USA 84:648-652.
Lemaitre, M., C. Bisbal, B. Bayard, and B. Lebleu (1987) "Biological activities of oligonuclotides linked to poly(L-lysine)," Nucleosides and Nucleotides 6:311-315.
Zuckerman, R., D. Corey, and P. Schultz (1987) "Effcient methods for attachment of thiol specific probes to the 3' ends of synthetic oligodeoxyribonucleotides," Nucl. Acids Res. 15:5305-5321.
Brown Johnnie R.
Clontech Laboratories, Inc.
Kunz Gary L.
LandOfFree
Multifunctional controlled pore glass reagent for solid phase ol does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Multifunctional controlled pore glass reagent for solid phase ol, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multifunctional controlled pore glass reagent for solid phase ol will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-383979