Multiepitopic immunometric assay

Details Multiepitopic immunometric assay Multiepitopic immunometric assay

1987-09-24

1991-04-30

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

43524077, 435975, 436531, 436535, 436548, 436800, 436804, 436868, 436819, 436813, 436814, 436815, C12N 500, G01N 3353, G01N 33545, C12Q 100

Patent

active

050117718

ABSTRACT:
The invention relates to an immunometric assay for a multivalent antigen in a sample which comprises forming a complex of the antigen together with multiple immobilized monoclonal antibodies against different epitopes of the antigen and with a detectably labeled soluble monoclonal antibody which is identical to one of the multiple immobilized antibodies. The labeled antibody associated with the complex is separated from the remaining soluble antibody and the detectably labeled antibody associated with the complex or unassociated with the complex is detected. Any one of the multiple immobilized monoclonal antibodies shows, by itself, substantially less binding towards the antigen in the immunometric assay, when used with itself or another monocolonal antibody in soluble labeled form, than when used with the multiple immobilized antibodies in combination.

REFERENCES:
patent: 4223002 (1980-09-01), Newman
patent: 4361647 (1982-11-01), Remington et al.
patent: 4376110 (1983-03-01), David et al.
patent: 4471058 (1984-09-01), Smith et al.
patent: 4474892 (1984-10-01), Murad
patent: 4486530 (1984-12-01), David et al.
patent: 4514505 (1985-05-01), Canfield et al.
patent: 4565687 (1986-01-01), Kjazaeli et al.
Bidurt, et al., J. Immunol 134, pp. 457-464, 1985.
Goldstein, et al, J. Inf. Diseases 147, pp. 829-837, 1983.
Henchal, et al, J. Virol 68(3), pp. 845-851, 1987.
Rugg et al, Clin. Chem. 32(10): 1844-1848 (1986).
Shimizu et al., Clin. Chem. 28(3): 546-547 (1982).
Caraux et al, J. Immunol. 134(2): 835-840 (1985).
Longhi et al., J. Immunol. Meth. 92(1): 89-95 (1986).
Bellet et al., J. Clin. Endoc. and Metab. 63(6): 1319-1327 (1986).
Armstrong et al., J. Clin. Endoc. and Metab. 59(5): 867-874 (1984).
Mizuchi et al., J. Immunol. Methods 74(1): 369-374 (1984).
Bellet et al., Endocrinol. 115(1): 330-336 (1984).
Brock, David J. H., et al., Clinica Chimica Acta 122: 353-358 (1982).
Porstmann, T., et al., Clinica Chimica Acta 135: 13-22 (1983).
Nomura, M., et al., Journal of Immunological Methods 58: 292-300 (1983).
Hunter, W. M., et al., Journal of Immunological Methods 50: 133-144 (1982).
Van Heyningen, V., et al., Journal of Immunological Methods 50: 123-131.
Nomura, M., et al., Journal of Immunological Methods 56:13-17 (1983).
Porstmann, B., et al,. Journal of Immunological Methods 66: 179-185 (1984).
Uotila, M., et al., Journal of Immunological Methods 42: 11-15 (1981).
Ehrlich, P. H., et al., Journal of Immunology 128: 2709-2713 (1982).
Ehrlich, P. H., et al, Journal of Immunology 131: 1906-1912 (1983).
Sterman, N., et al., The Lancet, 1983, 647-649.
Shapiro, A. I., et al., American Journal of Obstetrics and Gynecology 148: 72-76 (1984).
Stewart, M. C., et al., Journal of Endocrinology 98: 323-330 (1983).
Bosch, A. M. G., et al., Protides of the Biological Fluids, vol. 29, pp. 837-842 (1982).
Schroeder, J., et al., Pregnancy Proteins: Biological, Chemical and Clinical Applications, pp. 69-76 (1982).

Affiliated with

Also associated with

Rating

Multiepitopic immunometric assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Multiepitopic immunometric assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multiepitopic immunometric assay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-640075