Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1997-12-31
1999-11-30
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
4352523, 4353201, 435 691, 435366, 4352542, 536 235, 536 231, C12N 1510, C12N 1563, C12N 1512
Patent
active
059941308
ABSTRACT:
Compositions and methods are disclosed for improving the effectiveness of a chemotherapeutic regimen to eradicate multidrug-resistant transformed cells from the body of a mammal, preferably from the body of a human. The present disclosure capitalizes on the discovery of a novel multidrug-resistance associated protein (MRP), herein designated MRP-.beta.. The disclosed compositions include MRP-.alpha. nucleic acids, including probes and antisense oligonucleotides, MRP-.beta. polypeptides and antibodies, MRP-.beta. expressing host cells, and non-human mammals transgenic or nullizygous for MRP-.beta.. The disclosed methods include methods for attenuating aberrant MRP-.beta. gene expression, protein production and/or protein function. In addition, methods are disclosed for identifying and using a modulator, such as an inhibitor, of MRP-.beta.. Preferably, the modulator is a small molecule.
REFERENCES:
patent: 5104858 (1992-04-01), Hait et al.
patent: 5182293 (1993-01-01), Sunkara et al.
patent: 5190946 (1993-03-01), Ramu et al.
patent: 5190957 (1993-03-01), Sunkara et al.
patent: 5262311 (1993-11-01), Pardee et al.
patent: 5369009 (1994-11-01), Arceci et al.
patent: 5387685 (1995-02-01), Powell et al.
patent: 5403574 (1995-04-01), Piwnica-Worms
patent: 5407653 (1995-04-01), Piwnica-Worms
patent: 5489519 (1996-02-01), Deeley et al.
patent: 5510239 (1996-04-01), Baracchini, Jr. et al.
patent: 5523304 (1996-06-01), Sunkara
patent: 5543423 (1996-08-01), Zelle et al.
patent: 5550149 (1996-08-01), Powell et al.
patent: 5556856 (1996-09-01), Engel et al.
patent: 5561141 (1996-10-01), Powell et al.
patent: 5599672 (1997-02-01), Liang et al.
Hamilton et al. (1984), "Experimental Model Systems of Ovarian Cancer: Applications to the Design and Evaluation of New Treatment Approaches," 11 Semin. Oncol. 3:285-298.
Batist et al. (1986), "Overexpression of a Novel Anionic Gluthathione Transferase in Multidrug-Resistant Human Breast Cancer Cells," 261 J. Biol. Chem. 33:15544-15549.
Marsh et al. (1986), "Isolation and Characterization of Adriamycin-resistant HL-60 Cells Which Are Not Defective In The Initial Intracellular Accumulation of the Drug," 46 Cancer Res. 4053-4057.
Mirski et al. (1987), "Multidrug Resistance in a Human Small Cell Lung Cancer Cell Line Selected in Adriamycin," 47 Cancer Res. 2594-2598.
Hyde et al. (1990), "Structural Model of ATP-binding Proteins Associated With Cystic Fibrosis, Multidrug Resistance and Bacterial Transport," 346 Nature 362-365.
Krishan (1990), "Rapid Determination of Cellular Resistance-Related Drug Efflux in Tumor Cells," 33 Meth. Cell Biol. 491-500.
Cole et al. (1992), "Overexpression of a Transporter Gene in a Multidrug-Resistant Human Lung Cancer Cell Line," 258 Science 1650-1654.
Higgins (1992), "ABC Transporters: From Microorganisms to Man," 8 Annu. Rev. Cell Biol. 67-113.
Cole (1993), "Multidrug Resistance-Associated Protein: Sequence Correction," 260 Science 879.
Egholm et al. (1993), "PNA Hybridizes to Complementary Oligonucleotides Obeying the Watson-Crick Hydrogen-Bonding Rules," 365 Nature 566-568.
Zaman et al. (1993), "Analysis of the Expression of MRP, the Gene For A New Putative Transmembrane Drug Transporter, in Human Multidrug Resistant Lung Cancer Cell Lines," 53 Cancer Res. 1747-1750.
Cole et al. (1994), "Pharmacological Characterization of Multidrug Resistant MRP-Transfected Human Tumor Cells," 54 Cancer Res. 5902-5910.
Ravnik-Glavac et al. (1994), "Sensitivity of Single-Strand Conformation Polymorphism and Heteroduplex Method For Mutation Detection in The Cystic Fibrosis Gene," 3 Human Mol. Gen. 5:801-.
Slapak et al. (1994), "Expression of the Multidrug Resistance Associated Protein and P-Glycoprotein in Doxorubicin-Selected Human Myeloid Leukemia Cells," 84 Blood 9:3113-3121.
Zaman et al. (1994), "The Human Multidrug Resistance-Associated Protein MRP is a Plasma Membrane Drug-Efflux Pump," 91 Proc. Nat'l. Acad. Sci. USA 8822-8826.
Almquist et al. (1995), "Characterization of the M.sub.r 190,000 Multidrug Resistance Proteins (MRP) in Drug-Selected and Transfected Humor Tumor Cells," 55 Cancer Res. 120-110.
Lautier et al. (1996), "Multidrug Resistance Mediated by the Multidrug Resistance Protein (MRP) Gene," 52 Biochem. Pharmacol. 967-977.
Perry-O'Keefe et al. (1996), "Peptide Nucleic Acid Pre-Gel Hybridization: An Alternative to Southern Hybridization," 93 Proc. Natl. Acad. Sci. USA 14670-14675.
Ruetz et al. (1996), "Functional Expression of the Multidrug Resistance-Associated Protein in the Yeast Saccharomyces cerevisiae," 271 J. Biol. Chem. 8:4154-4160.
Stewart et al. (1996), "Reduction of Expression of the Multidrug Resistance Protein (MRP) in Human Tumor Cells by Antisense Phosphorothioate Oligonucleotides," 51 Biochem. Pharmacol. 461-469.
Stride et al. (1996), "Structure and Expression of the Messenger RNA Encoding the Murine Multidrug Resistance Protein, and ATP-Binding Cassette Transporter," 49 Mol. Pharmacol. 962-971.
Sager (1997), "Expression Genetics in Cancer: Shifting the Focus From DNA to RNA," 94 Genetics 952-955.
Mark D. Adams et al., Nature 377 (Supp):3-17, Sep. 28, 1995.
Rando Allikmets et al., Hum. Mol. Genet. 5 (10):1649-1655, Mar. 26, 1997.
L. Hillier et al., WashU-Merck EST Project, Genbank Accession No. H17207, Jun. 29, 1995.
Suzuki, T., et al., "cDNA cloning of a short type of multidrug resistance protein homologue, SMRP, from a human lung cancer cell line" Biochemical and Biophysical Research Communications 238(3):790-4 (1997).
Kool, M., et al., "Four homologs of the multidrug resistance protein MRP1" Eighty-Eighth Annual Meeting of the American Association for Cancer Research, San Diego, CA, Apr. 12-15, 1997, Proceedings of the American Association for Cancer Research Annual Meeting, 38(0), (1997), abstract#2941.
Genbank.RTM. Accession No. H17207 for ym42a06.rl Soares infant brain 1NIB Homo sapiens cDNA clone 50857 5' similar to SP:MRP.sub.- Human P33527 Multidrug Resistance-Associated; mRNA sequence.
Hutson Richard
Millennium Pharmaceuticals Inc.
Prouty Rebecca E.
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