Multi-well single-membrane permeation device and methods

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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4352884, 4352885, 4352975, 422101, 436178, 436809, C12Q 100

Patent

active

060430273

ABSTRACT:
The invention provides exemplary testing devices, systems, and methods for evaluating the permeation of various chemicals through different types of cells. In one exemplary embodiment, a testing device is provided which comprises a base member and a top member having a plurality of wells which are aligned when the top member is secured to the base member. A membrane sheet which includes at least one layer of cells grown on the sheet is placed between the base member and the top member prior to assembly. Test samples are placed into the wells in the top member and samples are removed from the top and bottom wells at a later time and tested to determine the amount of test sample which permeated through the cells.

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