Multi-well bone culture device for use in assessment of bone cel

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

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4352992, 4353051, 4353052, 435326, 435372, 435402, C12M 300

Patent

active

058613064

ABSTRACT:
The present invention describes a multi-well bone cell culture device for use in the quantitative and/or qualitative assessment of bone cell activity, the device comprising a flat base having a sintered film of calcium phosphate entities on one side and an open-ended multi chamber unit positioned on top of the film which by a sealing means is sealed to the film coated base forming individual containment wells. The device is useful in the analysis of bone cell function, for the screening and determination of bone cell disease, and the development of drugs to alter bone cell activity.

REFERENCES:
patent: 3726764 (1973-04-01), White
patent: 3745091 (1973-07-01), McCormick
"Failure of Cells of the Mononuclear Phagocyte Series to Resorb Bone", Chambers, et al., Calcified Tissue International, 1984, pp. 556-558.
"The resorption of biological and non-biological substrates by cultured avian and mammalian osteoclasts", Jones, et al., Anatomy and Embryology, 1984, pp. 247-256.
"Resorption of Dentine by Isolated Osteoclasts in vitro", Boyde, et al., Br. Dent J. 1984, pp. 216-220.
"The effect of substrate composition and condition on resorption by isolated osteoclasts", Shimizu, et al., Bone and Mineral, 6 (1989), pp. 261-275.
"An Assay System Utilizing Devitalized Bone for Assessment of Differentiation of Osteoclast Progenitors", Amano, et al., Journal Of Bone And Mineral Research, vol. 7 No. 3, Mar. 1992, pp. 321-327.
"Resorption Of Bone By Isolated Rabbit Osteoclasts", Chambers, et al., J. Cell. Sci. 66, 1984, pp. 383-399.

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