Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing element or inorganic compound except carbon dioxide
Reexamination Certificate
2003-10-01
2010-06-08
Carlson, Karen Cochrane (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing element or inorganic compound except carbon dioxide
C435S252100, C435S257100
Reexamination Certificate
active
07732174
ABSTRACT:
A method of using sequential chemostat culture vessels to provide continuous H2production, in which photosynthetic O2evolution and H2photoproduction are separated physically into two separate bioreactors, comprising: a) growing a microorganism culture able to continuously generate H2by photosynthetically producing cells at about the early-to-late log state in a first photobioreactor operating as a sulfur chemostat under aerobic and/or conditions; b) continuously feeding cells from the first photobioreactor to a second photobioreactor operating under anaerobic conditions and sulfur deprivation conditions resulting from constant uptake of sulfate in the first bioreactor and a low rate of culture flow between the first and second bioreactors, for induction of hydrogenase and H2photoproduction to allow for continuous cultivation of the microorganism's cells in the first photobioreactor and constant H2production in the second photobioreactor, and c) H2gas from the second photobioreactor.
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Kosourov, Sergey et al., “Sustained Hydrogen Photoproduction byChlamydomonas reinhardtii; Effects of Culture Parameters.” Biotechnology and Bioengineering. June 2002, vol. 78, No. 7, pp. 731-740, see entire document.
Ghirardi Maria L.
Kosourov Sergey
Seibert Michael
Alliance For Sustainable Energy LLC
Carlson Karen Cochrane
Kosson Rosanne
Stolpa John C.
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