Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1986-04-25
1989-02-07
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435301, 435805, 435810, 422 56, 436809, G01N 3353
Patent
active
048031542
ABSTRACT:
An assay for an immunologically active substance (B) in a biologically derived test sample comprising using an immuno-active membrane of a hydrophobic polymer sheet having numerous hydrophilic spots, on which a prefixed amount of an immuno-active substance (A) or (B) has been immobilized, and a coloring-membrane having numerous spots which contain a color-producing reagent being capable of developing color by the catalytic effect of an enzyme at the corresponding positions to those of the immuno-active membrane, and making contact the immuno-active membrane with said test sample in the presence of an enzyme-labeled immuno-active substance (A') or (B') and a substrate for reacting the substance (A), substance (B) and enzyme-labeled substance (A') or (B') competitively or non-competitively, and superposing the coloring-membrane of on the immuno-active membrane, followed by colorimetrically measuring the resulting color and thereby determining the amount of the substance (B) in the test sample.
REFERENCES:
patent: 3853468 (1974-12-01), Haymond
patent: 4055394 (1977-10-01), Friedman et al.
patent: 4157895 (1979-06-01), Finlay et al.
patent: 4305721 (1981-12-01), Bernstein
patent: 4357142 (1982-11-01), Schall et al.
patent: 4591570 (1986-05-01), Chang
Horiguchi Kazuo
Kaku Shigeru
Nakanishi Setsuko
Nishimura Masaji
Uo Junko
Rosen Sam
Shionogi & Co. Ltd.
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