Multi-purpose blood diluent for use in electronic blood analysis

Compositions – Nonreactive analytical – testing – or indicating compositions

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23230B, 424 2, 424 3, 424254, 424258, 424310, 435 4, G01N 3316, C09K 300

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active

042448379

ABSTRACT:
A multi-purpose blood diluent especially suitable for use in electronic enumeration and sizing of blood cells, determination of hemoglobin concentration and their collective indices and platelet parameters in a single blood sample by means of suitable electronic instrumentation. The diluent is characterized as azide-free and capable of affording accurate, reproducible hematological test results from the said suitable electronic instrumentation. The diluent employs a chelating agent which functions as a bacteriostat and fungistat agent to prevent adverse growth of microorganisms, methohexital sodium barbituate as a compatible stabilizing agent for maintaining desired morphology of red blood cells, and an inorganic metallic sulfate for suppressing turbidity caused by abnormal globulins and/or high leukocyte counts which are encountered in certain blood samples. Because of the capability for use of the diluent in platelet determinations by the instrumentation, the diluent further is free of detergent action which would cause bubbles capable of interfering with the enumeration of platelets by the instrumentation. A modified and adjusted embodiment of the diluent enables its use in veterinary laboratory testing applications.

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