Multi-parameter fluorimetric analysis in a massively...

Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation

Reexamination Certificate

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C250S458100

Reexamination Certificate

active

10688101

ABSTRACT:
A method for analyzing properties of a sample by measuring fluorescence parameters in multiple foci, comprises the steps of splitting a collimated primary laser beam with a splitting device into at least two collimated secondary laser beams and deflecting the secondary laser beams such that they propagate at different propagation angles with respect to an optical axis of a focussing optic, focussing the secondary laser beams with the focussing optic into at least two volume elements in the sample, detecting light emitted from the volume elements with a detecting device, and evaluating the detected light for obtaining the properties to be analyzed. Furthermore, a device for implementing this method is described. The invention is particularly suited for high-throughput screening applications.

REFERENCES:
patent: 5252834 (1993-10-01), Lin
patent: 6229635 (2001-05-01), Wulf
patent: 6262423 (2001-07-01), Hell et al.
patent: 6455861 (2002-09-01), Hoyt
patent: 6603537 (2003-08-01), Dietz et al.
patent: 2002/0070349 (2002-06-01), Hoyt
patent: 0 424 934 (1991-05-01), None
patent: WO 00/11024 (2000-03-01), None
patent: WO 02/14838 (2002-02-01), None
Abstract of Japan, 2002005834, Kenji, “Distribution Measuring Apparatus for Fluorescence Labeled Substance,” (Jan. 9, 2002).

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